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| Lovatto,Naglezi de Menezes; Loureiro,Bruno Bianchi; Bender,Ana Betine Beutinger; Loureiro,Cristiane Bianchi; Goulart,Fernanda Rodrigues; Speroni,Caroline Sefrin; Macagnan,Fernanda Teixeira; Piana,Mariana; Silva,Leila Picolli da. |
| ABSTRACT: Response surface methodology was used to determine optimum conditions for extraction of protein from pumpkin seed meal. A central composite rotational design (CCRD) including independent variables such, pH and sodium trimetaphosphate (STMP) content was used. Maximum yield was obtained at a central point of CCRD, with STMP content and pH of 4% and 4.5, respectively. These conditions resulted in protein yield of 50.04 g of soluble protein from extract/100 g pumpkin seed. The optimization of pH and STMP content allowed obtaining a product with a high protein concentration (62.56 g 100 g-1), digestibility (62.03 g 100 g-1) and concentration of essential amino acids (27.26 g 100 g-1). Regarding the polyphenols concentration, phosphorylated protein... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amino acids; Central composite rotational design; In vitro digestibility; New ingredient. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000200751 |
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