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| Spilki,Fernando R.; Franco,Ana C.; Rijsewijk,Frans A. M.; Weiblen,Rudi; Flores,Eduardo F.; Roehe,Paulo M.. |
| This study aimed the in vitro growth characterization of a previously constructed Brazilian bovine herpesvirus 1.2a with a deletion in the glycoprotein E gene (BHV-1.2a gE-). The plaque sizes, penetration and growth kinetics of the Brazilian BHV-1.2a gE- were studied and compared with the parental virus, as well as with a BHV-1.1 gE- recombinant derived from an European BHV-1.1 strain. No statistical differences were observed between the gE- recombinants and the respective parental viruses penetration assays were performed. When single step growth curves were studied, no statistical differences were observed between gE- and parental viruses. However, it was observed that both gE- viruses were excreted from cells in significantly higher titres at 11 hours... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis; Differential vaccine; In vitro growth characteristics; GE- recombinant; BHV-1.2a; Bovine herpesviruses. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200017 |
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| Luz,Roger B.; Staggemeier,Rodrigo; Fabres,Rafael B.; Soliman,Mayra C.; Souza,Fernanda G.; Gonçalves,Raoni; Fausto,Ivone V.; Rigotto,Caroline; Heinzelmann,Larissa S.; Henzel,Andréia; Fleck,Juliane D.; Spilki,Fernando R.. |
| Adenoviruses are among the most promising viral markers of fecal contamination. They are frequently found in the water, sediment and soil of regions impacted by human activity. Studies of the bioaccumulation of enteric viruses in shrimp are scarce. The cities located in the northern coast of the lake systems in Southern Brazil have high urbanization and intensive farming rates, and poor sewage collection and treatment. One hundred (n = 100) Farfantepenaeus paulensis pink-shrimp specimens and 48 water samples were collected from coastal lagoons between June 2012 and May 2013. Water samples were concentrated and the shrimp, mashed. After DNA extraction, samples were analyzed by real time polymerase chain reaction (qPCR) in order to detect and quantify viral... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Adenovirus; Farfantepenaeus paulensis; Pink-shrimp; Water quality; Northern Coast of Rio Grande do Sul. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300715 |
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| Heck,Karina; De Marco,Évilin G.; Hahn,Ana B. B.; Kluge,Mariana; Spilki,Fernando R.; Van Der Sand,Sueli T.. |
| A técnica da compostagem é um recurso empregado com o objetivo de reciclar resíduos orgânicos domésticos e sanitizar o lodo produzido nas estações de tratamento de efluentes de esgoto (ETE) podendo-se obter um composto com propriedades de fertilizante agrícola e/ou corretor de solos degradados. A alta temperatura atingida pelo sistema deve ser responsável pela redução de micro-organismos patogênicos presentes no início do processo assegurando, desta forma, a qualidade microbiológica do composto sem oferecer riscos de contaminação, conforme preconiza a Resolução 354/2006 do CONAMA. Objetivou-se, com o trabalho, avaliar a influência da temperatura sobre a redução de Escherichia coli, Salmonella sp., ovos de helmintos e vírus entéricos durante o processo de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lodo de esgoto; Coliformes; Matéria orgânica. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662013000100008 |
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