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| Oliveira,Eder Silva de; Junges,Ângela; Sbaraini,Nicolau; Andreis,Fábio Carrer; Thompson,Claudia Elizabeth; Staats,Charley Christian; Schrank,Augusto. |
| Abstract Cell walls are involved in manifold aspects of fungi maintenance. For several fungi, chitin synthesis, degradation and recycling are essential processes required for cell wall biogenesis; notably, the activity of β-N-acetylglucosaminidases (NAGases) must be present for chitin utilization. For entomopathogenic fungi, such as Metarhizium anisopliae, chitin degradation is also used to breach the host cuticle during infection. In view of the putative role of NAGases as virulence factors, this study explored the transcriptional profile and evolution of putative GH20 NAGases (MaNAG1 and MaNAG2) and GH3 NAGases (MaNAG3 and MaNAG4) identified in M. anisopliae. While MaNAG2 orthologs are conserved in several ascomycetes, MaNAG1 clusters only with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: NAGases; GH20 and GH3; Metarhizium; Chitinolytic system; Entomopathogenesis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500843 |
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| Staats,Charley Christian; Boldo,Juliano; Broetto,Leonardo; Vainstein,Marilene; Schrank,Augusto. |
| Mycoplasmas are very fastidious in their nutritional requirements for in vitro growth and have limited biosynthetic capacity, a reflection of their reduced genomes. As a result, these bacteria depend upon external metabolites for nutrition and growth and have developed dependence on their hosts for survival and maintenance. Protein degradation and peptide importation play an important role in Mycoplasma spp. nutrition, and proteases can play a role in host adaptation and pathogenicity. Here, we present a general survey on the genes involved in protein degradation, secretion and importation, comparing all available Mollicute genomes. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycoplasma; Proteases; Minimal genomes; Secretion systems. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200009 |
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| Paula,Daphine Ariadne Jesus de; Silva,Lívia Kmetzsch Rosa e; Staats,Charley Christian; Vainstein,Marilene H.; Joanoni,Ana Lúcia Pinto; Nakazato,Luciano; Dutra,Valéria. |
| Cryptococcus neoformans and C. gattii are pathogenic yeasts that cause life-threatening diseases in humans and animals. Iron is an essential nutrient for virtually every organism as it functions as a cofactor in numerous essential enzymatic reactions. In the literature, the competition for iron between microbes and mammalian hosts during infection is well documented. In this study, we used representational difference analysis (RDA) in order to gain a better understanding of how C. gattii responds to iron starvation. A total of 15 and 29 genes were identified as having altered expression levels due to iron depletion after 3 h and 12 h, respectively. Of these, eight genes were identified in both libraries. The transcripts were related to many biological... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cryptococcus gattii; Iron; Representational difference analysis; Yeast. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300008 |
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