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การจัดการโรคใบขาวอ้อยด้วยการใช้พันธุ์ปลอดโรค Thai Agricultural
Nilubon Taweekul; Taksina Sansayawichai; Kanjana Kirasak; Suchirat Sakuanrungsirikul; Narautai Worastit; Peaingpen Sarawat.
Sugarcane white leaf disease caused by phytoplasma is a serious problem for sugarcane production in Thailand. The pathogen can mainly be transmitted by insect vectors such as leafhoppers, and through seed cane. Planting of disease free seed cane is an important method for reducing the disease. However, the plants may be re-infected by insect vectors. Therefore, the disease free seed cane utilization to decrease the disease was evaluated during 2006 - 2011. Sugarcane white leaf disease free seedlings were produced by apical meristem culture technique with phytoplasma detection by nested PCR method. The Seedlings were transplanted and multiplied in sugarcane planting areas mainly in Northeast of Thailand. Sugarcane white leaf phytoplasma incidence was...
Tipo: Collection Palavras-chave: Sugarcane; Phytoplasma apical meristem culture; SCWL phytoplasma disease free seed cane; White leaf; Seed cane; อ้อย; โรคใบขาวอ้อย; โรคและศัตรูพืช; พันธุ์ปลอดโรค; เชื้อไฟโตพลาสมา; การเพาะเลี้ยงเนื้อเยื่อ; ยอดอ่อน; การตรวจเชื้อสาเหตุโรค; ชีวโมเลกุล.
Ano: 2012 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5547
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ความหลากหลายทางพันธุกรรมของเชื้อไฟโตพลาสมาที่ก่อโรคในอ้อย และหญ้าบางชนิดของประเทศไทย จากลำดับนิวคลีโอไทด์บริเวณ 16S-23S rRNA intergenic spacer region Thai Agricultural
Suchirat Sakuanrungsirikul; Theerawut Wongwarat; Sunee Srisink; Piyada Theerakulpisut.
The phylogenetic analysis of phytoplasmas focused on the nucleotide sequences of the intergenic 16s-23s rRNA spacer region that primers enable the amplification of a 210 bp fragment by nested-PCR, are established for studying of genetic diversity of phytoplasma associated with diseases of sugarcane and some grasses in Thailand. In this present study, 99 phytoplasma infected sugarcane samples exhibiting various phenotypic symptoms and 15 samples of grasses showing white leaves were collected from many locations in Thailand and subjected for nucleotide sequencing. Phylogenetics tree based on 16S-23S ITS gene sequences showed two primary clusters: (1) phytoplasmas that cause diseases in sugarcane and (2) phytoplasmas that cause diseases in grasses. The...
Tipo: Collection Palavras-chave: Phytoplasma; Sequencing analysis; White leaves diseases; Genetic diversity; อ้อย; โรคใบขาว; ไฟโตพลาสมา; เชื้อสาเหตุโรค; ลำดับนิวคลีโอไทด์; ความหลากหลายทางพันธุกรรม.
Ano: 2012 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5551
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