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Experimental infections with Paracoccidioides brasiliensis obtained from armadillos: comparison to clinical isolates BJID
Nishikaku,Angela Satie; Peraçoli,Maria Terezinha S.; Bagagli,Eduardo; Sugizaki,Maria Fátima; Sartori,Alexandrina.
Paracoccidioides brasiliensis causes paracoccidioidomycosis (PCM) that is one of the most prevalent systemic human mycoses in Latin America. Armadillos show a high incidence of PCM infection and could, therefore, be a natural reservoir for this fungus. In this study were compared the virulence profiles of isolates obtained from nine-banded armadillos (Dasypus novemcinctus) (PbT1 and PbT4) and isolates from PCM patients (Pb265 and Bt83). Pathogenicity was evaluated by fungal load and analysis of colony morphology. Immunity against the fungus was tested by delayed type hypersensitivity test (DTH) and antibody quantification by ELISA. The higher virulence of PbT1 and PbT4 was suggested by higher fungal load in spleen and lungs. Armadillo isolates and Bt83...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Paracoccidioides brasiliensis; Armadillos; Infection; BALB/c mice.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000100013
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Comparison between qualitative and semiquantitative catheter-tip cultures: laboratory diagnosis of catheter-related infection in newborns BJM
Marconi,Camila; Cunha,Maria de Lourdes RS; Lyra,João C; Bentlin,Maria R; Batalha,Jackson EN; Sugizaki,Maria Fátima; Rugolo,Lígia MSS.
This prospective study evaluated semiquantitative and qualitative catheter-culture methods for diagnosis of catheter-related infection (CRI) in newborns. Catheter tips from newborns admitted to the Neonatal Unit of the University Hospital of the Botucatu Medical School, UNESP were included in the study. Catheter cultures were performed with both semiquantitative and qualitative techniques. For CRI diagnosis, microorganisms isolated from catheter cultures and from peripheral blood cultures were identified and submitted to agent susceptibility test. The gold standard was the certain CRI diagnosis when same microorganism (specie and profile of susceptibility to agents) was isolated from both catheter tips and peripheral blood culture. A total of 85 catheters...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Catheter-related infection; Catheter culture; Semiquantitative culture; Newborns; Coagulase-negative staphylococci.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200012
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Candida species biofilm and Candida albicans ALS3 polymorphisms in clinical isolates BJM
Bruder-Nascimento,Ariane; Camargo,Carlos Henrique; Mondelli,Alessandro Lia; Sugizaki,Maria Fátima; Sadatsune,Terue; Bagagli,Eduardo.
Over the last decades, there have been important changes in the epidemiology of Candida infections. In recent years, Candida species have emerged as important causes of invasive infections mainly among immunocompromised patients. This study analyzed Candida spp. isolates and compared the frequency and biofilm production of different species among the different sources of isolation: blood, urine, vulvovaginal secretions and peritoneal dialysis fluid. Biofilm production was quantified in 327 Candida isolates obtained from patients attended at a Brazilian tertiary public hospital (Botucatu, Sao Paulo). C. albicans ALS3 gene polymorphism was also evaluated by determining the number of repeated motifs in the central domain. Of the 198 total biofilm-positive...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida spp.; Biofilm; ALS3 gene.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400030
Registros recuperados: 3
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