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| Sumantha,Alagarsamy; Deepa,Paul; Sandhya,Chandran; Szakacs,George; Soccol,Carlos Ricardo; Pandey,Ashok. |
| Rice bran was used as the substrate for screening nine strains of Rhizopus sp. for neutral protease production by solid-state fermentation. The best producer, Rhizopus microsporus NRRL 3671, was used for optimizing the process parameters for enzyme production. Fermentation carried out with 44.44 % initial moisture content at a temperature of 30 C for 72 h was found to be the optimum for enzyme secretion by the fermenting organism. While most of the carbon supplements favored enzyme production, addition of casein resulted in a marginal increase in protease yield. Fermentation was then carried out under optimized conditions to obtain the crude extract of the enzyme, which was partially purified by precipitation and dialysis. A 3-fold increase in the enzyme... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Solid-state fermentation; Rice bran; Rhizopus; Neutral protease; Agro-industrial residues. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600019 |
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