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| Sun,Dao-Cai; Li,De-Hua; Ji,Hui-Cang; Rao,Guo-Zhou; Liang,Li-Hua; Ma,Ai-Jie; Xie,Chao; Zou,Gui-Ke; Song,Ying-Liang. |
| In order to understand the mechanisms of poor osseointegration following dental implants in type 2 diabetics, it is important to study the biological properties of alveolar bone osteoblasts isolated from these patients. We collected alveolar bone chips under aseptic conditions and cultured them in vitro using the tissue explants adherent method. The biological properties of these cells were characterized using the following methods: alkaline phosphatase (ALP) chemical staining for cell viability, Alizarin red staining for osteogenic characteristics, MTT test for cell proliferation, enzyme dynamics for ALP contents, radio-immunoassay for bone gla protein (BGP) concentration, and ELISA for the concentration of type I collagen (COL-I) in the supernatant.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alveolar bone; Cell culture; Implant; Osteoblast; Type 2 diabetics. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600005 |
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