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| Zhao,Jianglin; Wang,Xiaohan; Sun,Weibo; Mou,Yan; Peng,Youliang; Zhou,Ligang. |
| Background: Berkleasmium sp. Dzf12, an endophytic fungus from Dioscorea zingiberensis, was a high producer of palmarumycin C13 with various bioactivities. In the present study, the experimental designs based on statistics were employed to evaluate and optimize the medium for palmarumycin C13 production in mycelia liquid culture of Berkleasmium sp. Dzf12. Results: Among various carbon and nitrogen sources, glucose, peptone and yeast extract were found to be the most favourable for palmarumycin C13 production based on the one-factor-at-a-time experiments. After Plackett-Burman test on the medium, glucose, peptone and yeast extract were further verified to be the most significant factors to stimulate palmarumycin C13 accumulation. These three factors (i.e.,... |
| Tipo: Journal article |
Palavras-chave: Berkleasmium sp. Dzf12; Central composite design; Endophytic fungus; Mycelia liquid culture; Palmarumycin C13; Plackett-Burman test; Response surface methodology. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600016 |
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| Li,Peiqin; Luo,Haiyu; Meng,Jiajia; Sun,Weibo; Wang,Xiaohan; Lu,Shiqiong; Peng,Youliang; Zhou,Ligang. |
| Background Three oligosaccharides (EOS, WOS and SOS) were respectively prepared from the corresponding polysaccharides, namely exopolysaccharide (EPS), water-extracted mycelial polysaccharide (WPS) and sodium hydroxide-extracted mycelial polysaccharides (SPS) from the endophytic fungus Fusarium oxysporum Dzf17. In this study, the effects of EOS, WOS and SOS on the activities of the defense-related enzymes, namely phenylalanine ammonia lyase (PAL), polyphenoloxidase (PPO) and peroxidase (POD) in its host plant Dioscorea zingiberensis cultures were investigated. Results For the suspension cell cultures of D. zingiberensis, the highest PAL activity was induced by 0.5 mg/mL of WOS at 48 h after treatment, which was 4.55-fold as that of control. Both PPO and... |
| Tipo: Journal article |
Palavras-chave: Endophytic fungus; Phenylalanine ammonia lyase; Peroxidase; Polyphenoloxidase; Polysaccharide. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000400003 |
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