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ปัจจัยต่างๆ ที่มีผลต่อการถ่ายยีนเข้าสู่ปาล์มน้ำมัน Thai Agricultural
Potjamarn Suraninpong; Supawadee Tawaro; Sompong Te-chato.
Oil palm is a highly producing oil plant. The most cultivation area is in the southern part of Thailand. Expended area of oil palm in the others part needs suitable variety. Thus, the object of this study was to improvement of oil palm via gene transfer technique. Embryogenic callus inducing from young leaves of high yield oil palm were cultured on various kinds and concentrations of antibiotics for screening of suitable concentration using for gene transformation. The result showed that hygromycin, kanamycin and phosphinotricin at 20, 200 and 5 mg/l, respectively, were suitable for transformed selection. Cefotaxime concentration at 200 mg/l was suitable for killing of agrobacterium. The study of inoculation time, co-culture time and co-culture...
Tipo: Collection Palavras-chave: Agrobacterium tumefaciens; Oil palm; Gene transfer; Embryogenic callus; Antibiotic; ปาล์มน้ำมัน; การถ่ายยีน; สารปฏิชีวนะ; เอ็มบริโอเจนิคแคลลัส; ระดับความเข้มข้น; แถบดีเอ็นเอ; ใบอ่อน.
Ano: 2011 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5166
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