| An in vitro study was conducted to investigate the effect of different concentrations of Euglena (Euglena gracilis) on CH4 production, dry matter (DM) digestibility, volatile fatty acid (VFA) and ammonia N(NH3-N) concentration as well as on the protozoa population. The treatments considered were Euglena at concentrations of 0.0, 50, 100, 200, 400 and 1000 g/kg dry matter (DM) of the substrate (60:40 forage: concentrate ratio) incubated for 24 and 96 h using an in vitro continuous gas production and in vitro two-stage digestion procedure, respectively. The data were subjected to polynomial regression analysis. Methane emissions (ml/g DM) decreased at an increasing rate, generally with increasing concentration of Euglena but also exhibited quadratic (P<... |
