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| Shojaei,Taha Roodbar; Salleh,Mohamad Amran Mohd; Tabatabaei,Meisam; Ekrami,Alireza; Motallebi,Roya; Rahmani-Cherati,Tavoos; Hajalilou,Abdollah; Jorfi,Raheleh. |
| Mycobacterium tuberculosis, the causing agent of tuberculosis, comes second only after HIV on the list of infectious agents slaughtering many worldwide. Due to the limitations behind the conventional detection methods, it is therefore critical to develop new sensitive sensing systems capable of quick detection of the infectious agent. In the present study, the surface modified cadmium-telluride quantum dots and gold nanoparticles conjunct with two specific oligonucleotides against early secretory antigenic target 6 were used to develop a sandwich-form fluorescence resonance energy transfer-based biosensor to detect M. tuberculosis complex and differentiate M. tuberculosis and M. bovis Bacille Calmette–Guerin simultaneously. The sensitivity and specificity... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacterium tuberculosis complex (MTBC); Cadmium-telluride quantum dots (CdTe-QDs); Gold nanoparticles (AuNPs); Fluorescence resonance energy transfer (FRET); Sandwich-form FRET-based biosensor. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000600600 |
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| Tabatabaei,Meisam; Zakaria,Mohd Rafein; Rahim,Raha Abdul; Wright,André-Denis G.; Shirai,Yoshihito; Abdullah,Norhani; Sakai,Kenji; Ikeno,Shinya; Mori,Masatsugu; Kazunori,Nakamura; Sulaiman,Alawi; Hassan,Mohd Ali. |
| 16S ribosomal RNA (rRNA)-targeted fluorescent in situ hybridization combined with polymerase chain reaction (PCR)-cloning, light microscopy using Gram stains, scanning electron microscopy and denatured gradient gel electrophoresis were used to reveal the distribution of methanogens within an anaerobic closed digester tank fed with palm oil mill effluent. For specific detection of methanogens, 16S rRNA-cloning analysis was conducted followed by restriction fragment length polymorphism (RFLP) for presumptive identification of methanogens. To cover the drawbacks of the PCR-cloning study, the organization of the microorganisms was visualized in the activated sludge sample by using fluorescent oligonucleotide probes specific to several different methanogens,... |
| Tipo: Journal article |
Palavras-chave: Anaerobic digestion; DGGE; FISH; Methanogens; POME. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300012 |
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