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Selection of lactic yeast producing glucosylceramide from cheese whey OAK
Sugai, Michiko; Takakuwa, N; Ohnishi, Masao; Arai, Ikichi; Urashima, Tadasu; Oda, Yuji.
From 2150 isolates from raw milk and milk products, yeast strains were surveyed to produce glucosylceramide from cheese whey. Most of the 54 strains that had accumulated a detectable amount of glucosylceramide were identified as Kluyveromyces lactis var. lactis. The cells of K. lactis var. lactis strain M-11 derived from domestic raw milk accumulated glucosylceramide 2.5-fold higher than K. lactis var. lactis NBRC 1267, the reference strain selected from the culture collections. Strain M-16 of K. lactis var. lactis derived from the same origin was found to synthesize a considerable amount of steryl glucoside in addition to glucosylceramide. Sequence analysis of ribosomal DNA intergenic spacer two regions revealed that strains M-11 and M-16 were diverged...
Palavras-chave: Glucosylceramide; Steryl glucoside; Cheese whey; Kluyveromyces lactis var; Lactis.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1055
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Simultaneous Production of Sphingolipids and Ethanol by Kluyveromyces thermotolerans OAK
Tamura, M; Kimura, K; Yunoki, K; Matsumoto, O; Takakuwa, N; Oda, Yuji; Ohnishi, Masao; 小田, 有二; 大西, 正男.
Kluyveromyces thermotolerans strain NBRC 1674 was selected for the simultaneous production of sphingolipids and ethanol from beet molasses. The strain gradually synthesized ethanol with fermentation periods and attained a level slightly higher than that of the strain of Saccharomyces cerevisiae usually used for ethanol production. The sphingolipids accumulated in the cells were composed of almost equal amounts of free ceramides and glucosylceramides. The sphingoid bases and fatty acids of the two sphingolipids differed from each other and changed under aerobic and anaerobic growth conditions. Oxygen limitation may cause accumulation of sphinganine by inhibiting sphingolipid desaturases and enhance its proportion in both the sphingolipids.
Palavras-chave: CANDIDA-LIPOLYTICA YEAST; SACCHAROMYCES-CEREVISIAE; CEREBROSIDES; IDENTIFICATION; FERMENTATIONS; DESATURASE; PLANTS.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/692
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