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Land-use systems affect Archaeal community structure and functional diversity in western Amazon soils Rev. Bras. Ciênc. Solo
Navarrete,Acácio Aparecido; Taketani,Rodrigo Gouvêa; Mendes,Lucas William; Cannavan,Fabiana de Souza; Moreira,Fatima Maria de Souza; Tsai,Siu Mui.
The study of the ecology of soil microbial communities at relevant spatial scales is primordial in the wide Amazon region due to the current land use changes. In this study, the diversity of the Archaea domain (community structure) and ammonia-oxidizing Archaea (richness and community composition) were investigated using molecular biology-based techniques in different land-use systems in western Amazonia, Brazil. Soil samples were collected in two periods with high precipitation (March 2008 and January 2009) from Inceptisols under primary tropical rainforest, secondary forest (5-20 year old), agricultural systems of indigenous people and cattle pasture. Denaturing gradient gel electrophoresis of polymerase chain reaction-amplified DNA (PCR-DGGE) using the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Soil microbial ecology; Soil microbiology; Microbial diversity; Land use changes; Tropical soils.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832011000500007
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Endo-and exoglucanase activities in bacteria from mangrove sediment BJM
Soares Júnior,Fábio Lino; Dias,Armando Cavalcante Franco; Fasanella,Cristiane Cipola; Taketani,Rodrigo Gouvêa; Lima,André Oliveira de Souza; Melo,Itamar Soares; Andreote,Fernando Dini.
The mangrove ecosystem is an unexplored source for biotechnological applications. In this unique environment, endemic bacteria have the ability to thrive in the harsh environmental conditions (salinity and anaerobiosis), and act in the degradation of organic matter, promoting nutrient cycles. Thus, this study aimed to assess the cellulolytic activities of bacterial groups present in the sediment from a mangrove located in Ilha do Cardoso (SP, Brazil). To optimize the isolation of cellulolytic bacteria, enrichments in two types of culture media (tryptone broth and minimum salt medium), both supplemented with 5% NaCl and 1% of cellulose, were performed. Tests conducted with the obtained colonies showed a higher occurrence of endoglycolytic activity (33...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cellulose endo-1; 4-β-D-glucanase exo-1; 4-β-D-glucanase salinity.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300048
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Use of molecular approach to verify the influence of a eutrophic lagoon in the nearby ocean's bacterioplankton communities BJM
Taketani,Rodrigo Gouvêa; Araujo,Fábio Vieira; Weerelt,Margaretha Denise Maria van; Sanchez-Nunez,Maria Leonisa; Aniszewski,Erick; Rosado,Alexandre Soares.
Rodrigo de Freitas lagoon is an eutrophic aquatic environment. The waters from the lagoon are released to the sea at Ipanema and Leblon beaches, through Jardim de Alah channel. In this work, the influence of these waters on the bacterial communities of these beaches was investigated. Eleven sampling stations were set between the lagoon and the beaches, and the samples were analyzed by molecular and microbiological parameters. PCR-DGGE of the DNA extracted from the samples was performed using rpoB primers. Preliminary results indicate that all used approaches could reveal the influence of the lagoon on the beaches bacterial communities.
Tipo: Info:eu-repo/semantics/article Palavras-chave: DGGE; Diversity; Bacterioplankton; Eutrophic lagoon.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500017
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