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Sequence and expression pattern of the germ line marker vasa in honey bees and stingless bees Genet. Mol. Biol.
Tanaka,Érica Donato; Hartfelder,Klaus.
Queens and workers of social insects differ in the rates of egg laying. Using genomic information we determined the sequence of vasa, a highly conserved gene specific to the germ line of metazoans, for the honey bee and four stingless bees. The vasa sequence of social bees differed from that of other insects in two motifs. By RT-PCR we confirmed the germ line specificity of Amvasa expression in honey bees. In situ hybridization on ovarioles showed that Amvasa is expressed throughout the germarium, except for the transition zone beneath the terminal filament. A diffuse vasa signal was also seen in terminal filaments suggesting the presence of germ line cells. Oocytes showed elevated levels of Amvasa transcripts in the lower germarium and after follicles...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apis mellifera; Melipona quadrifasciata; Vasa; Social insect; Oogenesis.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300023
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