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Souza,Alessandra A. de; Takita,Marco A.; Coletta-Filho,Helvécio D.; Campos,Magnólia A.; Teixeira,Juliana E.C.; Targon,Maria Luísa P.N.; Carlos,Eduardo F.; Ravasi,Juliano F.; Fischer,Carlos N.; Machado,Marcos A.. |
The Citrus ESTs Sequencing Project (CitEST) conducted at Centro APTA Citros Sylvio Moreira/IAC has identified and catalogued ESTs representing a set of citrus genes expressed under relevant stress responses, including diseases such as citrus variegated chlorosis (CVC), caused by Xylella fastidiosa. All sweet orange (Citrus sinensis L. Osb.) varieties are susceptible to X. fastidiosa. On the other hand, mandarins (C. reticulata Blanco) are considered tolerant or resistant to the disease, although the bacterium can be sporadically detected within the trees, but no disease symptoms or economic losses are observed. To study their genetic responses to the presence of X. fastidiosa, we have compared EST libraries of leaf tissue of sweet orange Pêra IAC (highly... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ESTs; Citrus variegated chlorosis; Biotic stress; CitEST; Phytopathogen. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500024 |
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Berger,Irving J.; Freitas-Astúa,Juliana; Reis,Marcelo S.; Targon,Maria Luísa P.N.; Machado,Marcos A.. |
Out of the 18,942 flavedo expressed sequences (clusters plus singletons) in Citrus sinensis from the Citrus EST Project (CitEST), 25 were statistically supported to be differentially expressed in this tissue after a double in silico hybridization strategy against leaf-, flower-, and bark-derived ESTs. Five of them, two terpene synthases and three O-methyltransferases, are absent in the other citrus tissues with concomitant 2x2 statistics, supporting the hypothesis that they are putative flavedo-specific expressed sequences. The pattern of these differentially expressed sequences during fruit development suggests that most of them are developmentally regulated. Some expressed gene products, including a putative germin-like protein highly expressed in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: EST; Fruit; Gene expression; Orange; Tissue-specific. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500004 |
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