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| Rungrattanakasin,Budsayachat; Premjet,Siripong; Thanonkeo,Sudarat; Klanrit,Preekamol; Thanonkeo,Pornthap. |
| Abstract An intronless endoglucanase from thermotolerant Aspergillus fumigatus DBINU-1 was cloned, characterized and expressed in the yeast Kluyveromyces lactis. The full-length open reading frame of the endoglucanase gene from A. fumigatus DBiNU-1, designated Cel7, was 1383 nucleotides in length and encoded a protein of 460 amino acid residues. The predicted molecular weight and the isoelectric point of the A. fumigatus Cel7 gene product were 48.19 kDa and 5.03, respectively. A catalytic domain in the N-terminal region and a fungal type cellulose-binding domain/module in the C-terminal region were detected in the predicted polypeptide sequences. Furthermore, a signal peptide with 20 amino acid residues at the N-terminus was also detected in the deduced... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspergillus fumigatus; Cellulosic material; Gene cloning; Kluyveromyces lactis; Thermotolerant fungus. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300647 |
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| Chamnipa,Nuttaporn; Thanonkeo,Sudarat; Klanrit,Preekamol; Thanonkeo,Pornthap. |
| Abstract High potential, thermotolerant, ethanol-producing yeasts were successfully isolated in this study. Based on molecular identification and phylogenetic analysis, the isolated thermotolerant yeasts were clustered in the genera of Pichia kudriavzevii, Candida tropicalis, Candida orthopsilosis, Candida glabrata and Kodamea ohmeri. A comparative study of ethanol production using 160 g/L glucose as a substrate revealed several yeast strains that could produce high ethanol concentrations at high temperatures. When sugarcane bagasse (SCB) hydrolysate containing 85 g/L glucose was used as a substrate, the yeast strain designated P. kudriavzevii RZ8-1 exhibited the highest ethanol concentrations of 35.51 g/L and 33.84 g/L at 37 °C and 40 °C, respectively. It... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pichia kudriavzevii; Thermotolerant yeast; Ethanol production; Gene expression; Sugarcane bagasse hydrolysate. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200378 |
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