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Molecular evolution and transcriptional profile of GH3 and GH20 β-N-acetylglucosaminidases in the entomopathogenic fungus Metarhizium anisopliae Genet. Mol. Biol.
Oliveira,Eder Silva de; Junges,Ângela; Sbaraini,Nicolau; Andreis,Fábio Carrer; Thompson,Claudia Elizabeth; Staats,Charley Christian; Schrank,Augusto.
Abstract Cell walls are involved in manifold aspects of fungi maintenance. For several fungi, chitin synthesis, degradation and recycling are essential processes required for cell wall biogenesis; notably, the activity of β-N-acetylglucosaminidases (NAGases) must be present for chitin utilization. For entomopathogenic fungi, such as Metarhizium anisopliae, chitin degradation is also used to breach the host cuticle during infection. In view of the putative role of NAGases as virulence factors, this study explored the transcriptional profile and evolution of putative GH20 NAGases (MaNAG1 and MaNAG2) and GH3 NAGases (MaNAG3 and MaNAG4) identified in M. anisopliae. While MaNAG2 orthologs are conserved in several ascomycetes, MaNAG1 clusters only with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: NAGases; GH20 and GH3; Metarhizium; Chitinolytic system; Entomopathogenesis.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500843
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Contribution of WUSCHEL-related homeobox (WOX) genes to identify the phylogenetic relationships among Petunia species Genet. Mol. Biol.
Segatto,Ana Lúcia Anversa; Thompson,Claudia Elizabeth; Freitas,Loreta Brandão.
Abstract Developmental genes are believed to contribute to major changes during plant evolution, from infrageneric to higher levels. Due to their putative high sequence conservation, developmental genes are rarely used as molecular markers, and few studies including these sequences at low taxonomic levels exist. WUSCHEL-related homeobox genes (WOX) are transcription factors exclusively present in plants and are involved in developmental processes. In this study, we characterized the infrageneric genetic variation of Petunia WOX genes. We obtained phylogenetic relationships consistent with other phylogenies based on nuclear markers, but with higher statistical support, resolution in terminals, and compatibility with flower morphological changes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular phylogeny; Recently diverged species; Developmental genes.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400658
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