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Lema, Anais Kimberley; Clode, Peta L.; Kilburn, Matt R.; Thornton, Ruth; Willis, Bette L.; Bourne, David G.. |
Diazotrophic bacteria are instrumental in generating biologically usable forms of nitrogen by converting abundant dinitrogen gas (N-2) into available forms, such as ammonium. Although nitrogen is crucial for coral growth, direct observation of associations between diazotrophs and corals has previously been elusive. We applied fluorescence in situ hybridization (FISH) and nanoscale secondary ion mass spectrometry to observe the uptake of N-15-enriched diazotrophic Vibrio sp. isolated from Acropora millepora into conspecific coral larvae. Incorporation of Vibrio sp. cells was observed in coral larvae after 4-h incubation with enriched bacteria. Uptake was restricted to the aboral epidermis of larvae, where Vibrio cells clustered in elongated aggregations.... |
Tipo: Text |
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Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00664/77573/79543.pdf |
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