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Evaluation of MALDI-TOF mass spectrometry for the competitiveness analysis of selected indigenous cowpea (Vigna unguiculata L. Walp.) Bradyrhizobium strains from Kenya Organic Eprints
Mathu Ndungu, Samuel; Messmer, Monika M.; Ziegler, Dominik; Thuita, Moses; Vanlauwe, Bernard; Frossard, Emmanuel; Thonar, Cécile.
Cowpea N2 fixation and yield can be enhanced by selecting competitive and efficient indigenous rhizobia. Strains from contrasting agro-ecologies of Kilifi and Mbeere (Kenya) were screened. Two pot experiments were established consisting of 13 Bradyrhizobium strains; experiment 1 (11 Mbeere + CBA + BK1 from Burkina Faso), experiment 2 (12 Kilifi + CBA). Symbiotic effectiveness was assessed (shoot biomass, SPAD index and N uptake). Nodule occupancy of 13 simultaneously co-inoculated strains in each experiment was analyzed by matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS) to assess competitiveness. Strains varied in effectiveness and competitiveness. The four most efficient strains were further evaluated in a...
Tipo: Journal paper Palavras-chave: Crop health; Quality; Protection Africa.
Ano: 2018 URL: http://orgprints.org/33100/1/Ndungu-etal-2018-AMB-online-p1-14.pdf
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Cowpea (Vigna unguiculata L. Walp) hosts several widespread bradyrhizobial root nodule symbionts across contrasting agro-ecological production areas in Kenya Organic Eprints
Mathu Ndungu, Samuel; Messmer, Monika; Ziegler, Dominik; Gamper, Hannes; Mészáros, Éva; Thuita, Moses; Vanlauwe, Bernard; Frossard, Emmanuel; Thonar, Cécile.
Cowpea (Vigna unguiculata L. Walp.) is an important African food legume suitable for dry regions. It is the main legume in two contrasting agro-ecological regions of Kenya as an important component of crop rotations because of its relative tolerance to unpredictable drought events. This study was carried out in an effort to establish a collection of bacterial root nodule symbionts and determine their relationship to physicochemical soil parameters as well as any geographical distributional patterns. Bradyrhizobium spp. were found to be widespread in this study and several different types could be identified at each site. Unique but rare symbionts were recovered from the nodules of plants sampled in a drier in-land region, where there were also overall more...
Tipo: Journal paper Palavras-chave: Vegetables Crop health; Quality; Protection Africa.
Ano: 2018 URL: http://orgprints.org/33099/1/Ndungu-etal-2018-AEE-Vol261-p161-171.pdf
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