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| Inglis,Peter W.; Tigano,Myrian S.. |
| A phylogenetic analysis of the 5.8S rDNA and internal transcribed spacer (ITS1 and ITS2) sequences from some entomogenous Paecilomyces species supports the polyphyly of the genus and showed the existence of cryptic species. In the Eurotiales, anamorphs Paecilomyces variotii and Paecilomyces leycettanus were related to the teleomorphs Talaromyces and Thermoascus. In the Hypocreales, three major ITS subgroups were found, one of which included Paecilomyces viridis, Paecilomyces penicillatus, Paecilomyces carneus and isolates identified as Paecilomyces lilacinus and Paecilomyces marquandii. However, the majority of the P. lilacinus and P. marquandii isolates formed a distinct and distantly related subgroup, while the other major subgroup contained Paecilomyces... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paecilomyces spp.; Phylogenetic analysis; RDNA; Molecular taxonomy. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100025 |
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| Inglis,Peter W.; Tigano,Myrian S.; Valadares-Inglis,M. Cléria. |
| The entomopathogenic fungi Paecilomyces fumosoroseus and P. lilacinus have been transformed to resistance to the fungicide benomyl by a polyethylene glycol (PEG)-mediated procedure using a mutant <FONT FACE="Symbol">b</font>-tubulin gene from Neurospora crassa carried on plasmid pBT6. Benomyl-resistant transformants of P. lilacinus were obtained that could tolerate greater than 30 µg/ml benomyl and P. fumosoroseus transformants were obtained that could tolerate 20 µg/ml benomyl. Following 5 serial passages of transformants on benomyl-containing media and 5 serial passages on non-selective media, 100% of P. lilacinus transformants were found to be mitotically stable by a conidial germination test. In contrast, only 4 out of 9 transformants of P.... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000100023 |
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| Tavares,Eder T.; Tigano,Myrian S.; Mello,Sueli C. M.; Martins,Irene; Cordeiro,Celia M. T.. |
| Forty-nine Brazilian Dicyma pulvinata isolates were examined by morphological traits and RFLP, RAPD and AFLP analyses. This fungus is a mycoparasite of Microcyclus ulei, the causal agent of the most devastating rubber (Hevea brasiliensis) disease, known as "South American Leaf Blight" (SALB). These isolates were compared with an Indian isolate from Cercosporidium sp., and a French isolate from Cladosporium fulvum. They were also compared with Dicyma ampullifera from Papua New Guinea. The morphological parameters analyzed confirmed the identification of the Brazilian isolates. The graphic representations of the distance matrices of each molecular marker showed similar results. Dicyma pulvinata isolates from M. ulei were closely related, whereas the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fungi; Molecular markers; Biological control. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000200005 |
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| Luz,Christian; Silva,Ionizete G.; Magalhães,Bonifácio P.; Cordeiro,Celia M. T.; Tigano,Myrian S.. |
| Beauveria bassiana (Bals.) Vuill., isolate CG 306, was assayed against Triatoma infestans (Klug) using indirect application by contact with a treated filter paper. The fungus was deposited on filter paper by vacuum-filtration of a conidial suspension. Estimates of 50% survival time of insects were between 15 and 21 days at 3x10(6) and 10(7) conidia/cm² of treated support, respectively, without significant difference between concentrations. A concentration of 2.4x10(6) conidia/cm² of CG 306 was necessary to kill 50% of T. infestans third instar nymphs 25 days after inoculation at 25°C and 50% relative humidity, when insects were continuously exposed to conidia. The LC50 was significantly higher (2.0x10(7) conidia/cm²) when insects were exposed for 1 h to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Insecta; Chagas disease; Biological control; Hyphomycetes. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591999000100011 |
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| Silveira,Eni B.; Al-Janabi,Salah M.; Magalhães,Bonifácio P.; Carvalho,Luiz J.C.B.; Tigano,Myrian S.. |
| The genetic variability of 27 individuals from two distinct populations (Northeast and Central Brazil) of the grasshopper Schistocerca pallens (Thunberg) and eight isolates of its natural pathogen, the fungus Metarhizium flavoviride Gams & Rozsypal, was investigated using RAPD analysis. For the grasshoppers, ten different 10-mer oligonucleotide primers of arbitrary sequence were selected for analysis, resulting in 79 scorable binary characters. The program RAPDDIP applied to S. pallens revealed nucleotide diversity of 2.3 and 2.2% for the populations from Rio Grande do Norte (Northeast Brazil) and Federal District (Central Brazil), respectively. These values indicate the presence of high genetic variability within these populations. Conversely, the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Insecta; Genetic variability; Entomopathogenic fungus; Biological control; Molecular markers. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591998000100012 |
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