Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados Ciência e Agrotecnologia (2) Rev. Ciênc. Agron. (1) PAB (1) R. Bras. Zootec. (1) Autor Timbó,Ana Luiza de Oliveira (5) Davide,Lisete Chamma (4) Pereira,Roselaine Cristina (3) Pinto,José Eduardo Brasil Pereira (2) Souza Sobrinho,Fausto (2) Carvalho,Ana Cristina Portugal Pinto de (1) Morais,João Paulo Saraiva (1) Nunes,Juliane Dornellas (1) Pereira,Antônio Vander (1) Santos,Maurício Reginaldo Alves dos (1) Santos,Vanderley Borges dos (1) Souza Sobrinho,Fausto de (1) Souza,Patrícia Nirlane da Costa (1) Mais... Palavra-chave Forage (2) Brachiaria brizantha (1) Brachiaria ruziziensis (1) Casca de arroz carbonizada (1) Colchicine (1) Cytogenetics (1) DNA content (1) Flow cytometry (1) Forage plant breeding (1) Germplasm bank (1) Húmus de minhoca (1) In vitro cultivation (1) Interspecific hybrids (1) Nuclear DNA content (1) Number of chromosomes (1) Pennisetum glaucum (1) Pennisetum purpureum (1) Pollen viability (1) Polyploidy induction (1) Pó de casca de coco (1) Mais... Tipo do documento Info:eu-repo/semantics/article (4) Info:eu-repo/semantics/other (1) Ano 2014 (2) 2012 (1) 2010 (1) 2004 (1) País Brazil (5) Idioma Inglês (4) Português (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 5 Primeira ... 1 ... Última Avaliação de substratos e adubos orgânicos na aclimatização de plântulas de Heliconia psittacorum PAB Santos,Maurício Reginaldo Alves dos; Timbó,Ana Luiza de Oliveira; Carvalho,Ana Cristina Portugal Pinto de; Morais,João Paulo Saraiva. O objetivo deste trabalho foi avaliar a eficiência de três substratos orgânicos - casca de arroz carbonizada, pó de casca de coco seco e verde - e dois adubos -Vitasolo® e húmus de minhoca -, na aclimatização de plântulas de Heliconia psittacorum L., provenientes da micropropagação. Aos 75 dias, avaliaram-se a altura das plantas, o diâmetro do pseudocaule, o número de folhas e a área da terceira folha. A casca de arroz foi mais eficiente que o pó de casca de coco, verde ou seco; o pó de casca de coco verde foi mais eficiente que o pó de casca de coco seco; e o húmus foi mais eficiente que o Vitasolo®. Tipo: Info:eu-repo/semantics/other Palavras-chave: Húmus de minhoca; Casca de arroz carbonizada; Pó de casca de coco. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004001000014 Obtaining tetraploid plants of ruzigrass (Brachiaria ruziziensis) R. Bras. Zootec. Timbó,Ana Luiza de Oliveira; Souza,Patrícia Nirlane da Costa; Pereira,Roselaine Cristina; Nunes,Juliane Dornellas; Pinto,José Eduardo Brasil Pereira; Souza Sobrinho,Fausto de; Davide,Lisete Chamma. The objective of the present work was to induce chromosome doubling in genotypes of Brachiaria ruziziensis with potential for production of good-quality biomass and tolerance/resistance to stress caused by aluminum and spittlebugs. Germinated seeds of B. ruziziensis were immersed in 0.1% colchicine solution for 2 or 3 hours. Ploidy level was determined by flow cytometry and confirmed by chromosome count. Fertility of the tetraploid plants was assessed through evaluation of pollen viability by staining. There was no statistical difference with regard to seedling survival and number of duplicate seedlings between the treatments. Survival rate was 8%, of which 11.45% were tetraploid genotypes. The mean viability of pollen grains from the evaluated tetraploid... Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA content; Forage plant breeding; Number of chromosomes; Polyploidy induction; Pollen viability. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000300004 Nuclear DNA content and chromosome number in Brachiaria spp. genotypes Rev. Ciênc. Agron. Timbó,Ana Luiza de Oliveira; Pereira,Roselaine Cristina; Souza Sobrinho,Fausto; Davide,Lisete Chamma. Breeding programs for Brachiaria spp. use both intraspecies and interspecies crosses between sexual and apomictic plants in order to obtain new cultivars with the desired characteristics. As there are different ploidy levels both within and between species of this genus, it becomes necessary to evaluate the genotypes used in breeding programs, as a guide to breeders when adopting crossing strategies. In this work, DNA content and chromosome number were determined in order to characterise ploidy levels in Brachiaria spp. genotypes. In the analysis of 15 genotypes, DNA content varied with the ploidy levels (2x, 3x and 4x), and between species and/or taxon. The average DNA content was 1.74 pg (2x) in B. ruziziensis, 3.74 pg (4x) in B. decumbens and 3.52 pg... Tipo: Info:eu-repo/semantics/article Palavras-chave: Germplasm bank; Cytogenetics; Flow cytometry; Forage. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902014000100008 Protoplast production from napier grass and pearl millet triploid hybrids Ciência e Agrotecnologia Timbó,Ana Luiza de Oliveira; Davide,Lisete Chamma; Pinto,José Eduardo Brasil Pereira; Pereira,Antônio Vander. The objective of this work was to obtain protoplasts from napier grass and pearl millet triploid hybrids as a basis for future studies on chromosomal duplication. Explants were taken from mesophyll of in vitro- and in vivo-cultured plants or from calli of two triploid hybrids (H1 and H2), which were treated with enzymatic solutions containing different concentrations of cellulase R-10 (0.5, 1.0, 1.5 and 2.0%) with an additional 0.2% macerozyme and 0.1% driselase or 1.0% pectolyase Y-23 and 0.5% hemicellulase. Enzymatic digestion was monitored once every hour for five hours. Protoplasts were obtained from in vitro and in vivo leaflets of both triploid hybrids, and in vitro leaflets were the best explant sources. The quantity of produced protoplasts varied... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pennisetum purpureum; Pennisetum glaucum; In vitro cultivation; Interspecific hybrids; Forage. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000500020 Histogram score contributes for reliability of DNA content estimatives in Brachiaria spp Ciência e Agrotecnologia Timbó,Ana Luiza de Oliveira; Pereira,Roselaine Cristina; Santos,Vanderley Borges dos; Souza Sobrinho,Fausto; Davide,Lisete Chamma. Flow cytometry allows to estimate the DNA content of a large number of plants quickly. However, inadequate protocols can compromise the reliability of these estimates leading to variations in the values of DNA content the same species. The objective of this study was to propose an efficient protocol to estimate the DNA content of Brachiaria spp. genotypes with different ploidy levels using flow cytometry. We evaluated four genotypes (B. ruziziensis diploid and artificially tetraploidized; a tetraploid B. brizantha and a natural triploid hybrid), three buffer solutions (MgSO4, Galbraith and Tris-HCl) and three species as internal reference standards (Raphanus sativus, Solanum lycopersicum e Pisum sativum). The variables measured were: histogram score (1-5),... Tipo: Info:eu-repo/semantics/article Palavras-chave: Brachiaria ruziziensis; Brachiaria brizantha; Colchicine; Nuclear DNA content. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000600001 Registros recuperados: 5 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco