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Immunohistochemistry Analysis of Proteins Related with Apoptosis as Prognostic Factor inEpidermoid Carcinomaof Penis International Journal of Morphology
Novais,P. C; de Paula,A. A. P; Reis,R. B; Barione,D. F; Franqueiro,A. G; Rodrigues Jr,A. A; Lizarte,F. S. N; de Carvalho,C. A. M; Tirapelli,D. P. C; Peria,F. M; Saggioro,F. P; Tucci Jr,S; Cologna,A. J; Martins,A. C. P; Tirapelli,L. F.
The aim was to analyze the protein expression of apoptotic genes caspase-3, caspase-8 and bcl-2 with the immunohistochemistry technique, correlating with tumor grade (I, II and III) and with the patient survival in order to understand the basic mechanism of tumoral transformation. The immunohistochemistry reactions on 50 samples of squamous cell carcinoma were carried out with the avidin-biotin immunoperoxidase method and antigen recovery. The analyses were made using the graduation method "in crosses" (0 to 4 crosses - no stain to more than 75% of positives cells) and in categories (low, intermediate, high) of the cytoplasm immunoreactivity of the epidermoid penile carcinoma cells. It was observed a statistically significant difference when the expression...
Tipo: Journal article Palavras-chave: Bcl-2; Caspase-3; Caspase-8; Penile cancer; Apoptosis.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300043
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Analysis of the NMDA in Focal Cerebral Ischemia in Rats International Journal of Morphology
Tirapelli,D. P. C; Carlotti Jr,C. G; Leite,J. P; Lizarte,F. S. N; Tirapelli,L. F; Colli,B. O.
NMDAR (N-methyl-D-aspartate receptor) is one subtype of ionotrophic glutamate receptor which is extensively distributed in the central nervous system (CNS). In the mammalian CNS, NMDAR serves prominent roles in the pathophysiologic process of cerebral ischemia. This study aimed to investigate the pattern of expression of protein and gene of the excitatory neurotransmitter NMDAR in experimental focal cerebral ischemia and the hole of neuroprotection with hypothermia and ketoprofen. 120 rats were randomly divided into 6 groups (20 animals each): control - no surgery; sham - simulation of surgery; ischemic - focal ischemia for 1 hour, without reperfusion; ischemic + intraischemic hypothermia; ischemic + previous intravenous ketoprofen, and ischemic +...
Tipo: Journal article Palavras-chave: NMDA expression; Cerebral ischemia; Neuroprotection; Hypothermia and ketoprofen.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300035
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