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Overlapping of mononuclear cells derived from bone marrow in rats' intervertebral discs: an in vitro study Ciência Rural
Fontes,Erica Batista; Borges,Andréa Pacheco Batista; Pippi,Ney Luis; Rosa,Maurício; Sprada,Arícia; Togni,Monique.
In this study, bone marrow mononuclear cells (BM-MNC) derived from rats were used in order to promote intervertebral disc regeneration. These cells were isolated after centrifugation in a Ficoll-Paque™ PLUS density gradient and then placed in plastic dishes to proliferate during a period of 14 days. The BM-MNCs were previously labeled with the fluorescent membrane marker Chloromethyl-benzamidodialkylcarbocyanine (CM-DIL), and thereafter were implanted in rats' intervertebral discs explants as an in vitro experimental model. Daily analyses of the cells under a fluorescence microscope revealed morphological changes, which assumed a thin and elongated shape similar to cells that originally form the annulus fibroses. Histopathological analysis demonstrated the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mesenchymal stem cells; Intervertebral disc; In vitro; Rat.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000400024
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Ischemic myelopathy caused by fibrocartilaginous embolism in a horse Ciência Rural
Panziera,Welden; Bianchi,Ronaldo Michel; Pereira,Paula Reis; Flores,Mariana Martins; Togni,Monique; Pereira,Roberta Carneiro da Fontoura; Corte,Flávio De La; Fighera,Rafael Almeida.
ABSTRACT: This report described clinical, epidemiological, and pathological aspects of ischemic myelopathy caused by fibrocartilaginous embolism (FCE) in a 10-year-old, mixed breed gelding. Clinically, the horse presented acute hind limbs paralysis, with a clinical course of approximately 24 hours. At necropsy, no gross lesions were observed. Cross-sections of the spinal cord revealed focally extensive areas of malacia from the T10 to L4 segments. Focally extensive areas of liquefactive necrosis involving the gray matter and adjacent white matter were observed on histologic sections. The lumen of multiple blood vessels in the periphery of the necrotic areas was occluded by fibrocartilaginous emboli that strongly stained with alcian blue. Clinical signs,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Equine diseases; Neurological disorder; Ischemic myelopathy; Pathology.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200502
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