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Multidrug efflux systems in Escherichia coli and Enterobacter cloacae obtained from wholesome broiler carcasses BJM
Moreira,Maria Aparecida S.; Rodrigues,Patrícia P.C.F.; Tomaz,Rafael S.; Moraes,Célia A. de.
Members of the Enterobacteriaceae family are present in the intestines of man and animals as commensals or are important disease causing agents. Bacteria bearing multidrug efflux systems (MDR) are able to survive adverse ecological niches. Multiresistant Escherichia coli and Enterobacter cloacae isolates from wholesome broiler carcasses were investigated for the presence of MDR. Lowering of Minimal Inhibitory Concentration for antimicrobials in the presence of a proton-motive force (PMF) uncoupler was tested as a potential display of the MDR phenotype. PCR amplification of the genes encoding AcrA and AcrB, components of a MDR system was performed. Diversity of each species was ascertained by Pulsed-Field Gel Electrophoresis (PFGE) of DNA digested with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Multiresistance; Antimicrobials; Enterobacteriaceae; Proton-motive force; Diversity.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200007
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