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| Tomotani,Ester Junko; Vitolo,Michele. |
| Invertase, whether adsorbed on styrene-divinylbenzene copolymers or otherwise, was used for continuous sucrose hydrolysis using a cell-type membrane reactor (CTMR), coupled with an ultra (UF-100kDa), or a microfiltration (MF- pore diameter of 5 µm) membrane. In all tests, the pH (5.5), temperature (30 ºC), reaction volume (10 mL) and agitation (100 rpm) were set constant; whereas, variable parameters were: feeding rate (0.4, 0.8 and 1.6 h-1), inlet sucrose concentration (2.5, 6.5, 50 and 100 mM) and enzyme/resin ratio (1.64 mg or 3.28 mg of protein per 25, 50 or 100 mg of resin). The best result (yield of 100%, steady-state duration over 20h and specific reaction rate over 243 x 10-3 mmol/h.mE) was obtained when insoluble invertase (1.64 mg protein/100 mg... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sucrose; Invert sugar; Membrane reactor. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300022 |
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| Silva,Aline Ramos da; Tomotani,Ester Junko; Vitolo,Michele. |
| Conversion of sucrose into fructose and gluconic acid using invertase, glucose oxidase and catalase was studied by discontinuous (sequential or simultaneous addition of the enzymes) and continuous (simultaneous addition of the enzymes in a 100 kDa-ultrafiltration membrane reactor) processes. The following parameters were varied: concentration of enzymes, initial concentration of substrates (sucrose and glucose), pH, temperature and feeding rate (for continuous process). The highest yield of conversion (100%) was attained through the discontinuous (batch) process carried out at pH 4.5 and 37 ºC by the sequential addition of invertase (14.3 U), glucose oxidase (10,000 U) and catalase (59,000 U). |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sucrose/conversion; Fructose; Gluconic acid; Invertase; Glucose oxidase; Catalase. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200022 |
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