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Experimental Design Applied to the Optimization and Partial Characterization of Pectin Liase from a Newly Isolated Penicillium brasilianum BABT
Zeni,Jamile; Gomes,Jonaína; Ambroszini,Éllin; Basso,Ana Paula; Toniazzo,Geciane; Valduga,Eunice.
Penicillium brasilianum was previously isolated from tea and identified by molecular biology technique. A Plackett-Burman design, followed by a complete second order design was used for the screening of most important factors and to maximize the pectin liase (PMGL) activity, respectively. The maximum PMGL activity by P. brasilianum achieved was 9.0 U/mL after 48 h of cultivation in a medium containing pectin (33.0 g/L), yeast extract (30.0 g/L) and potassium phosphate (2.0 g/L) at 30ºC, with a stirring rate of 180 rpm, initial pH 5.5 and 5x106spores/mL inoculum size. The kinetic evaluation in terms of substrate consumption demonstrated that the maximum production of PMGL was at 72 h, and 40% of the total organic carbon, 25% of the nitrogen, 88% of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Penicillium brasilianum; Pectin liase; Experimental design technique.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600908
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Effect of Partial and Total Replacement of Inorganic by Organic Microminerals Sources on the Quality of Broiler Carcasses BABT
Lopes,Marcos; Paroul,Natalia; Barbosa,Juliana; Valduga,Eunice; Cansian,Rogerio Luis; Toniazzo,Geciane; Oliveira,Débora.
ABSTRACT Among the nutrients involved in the chicken diet, the microminerals deserve attention as they exert essential functions in the organism. These compounds can be provided in inorganic (traditional) and organic (chelate) forms. In organic form, the micromimerals can attend a new concept related to a better bio-availability. In this sense, the objective of this work was to evaluate the effect of the partial and total substitution of inorganic by organic sources of microminerals on the animal diet, assessing its concentration in the blood and liver after 21 and 40 days and also in the bones after 40 days. Moreover, the effect on the physical-chemical and sensory characteristics of the muscle after 15 days of storage at 4°C was also evaluated. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bio-availability; Broilers; Microminerals; Nutrition.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100440
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Decontamination of Pig Carcasses Using Water Pressure and Lactic Acid BABT
Brustolin,Jean Carlos; Dal Pisol,Andreia; Steffens,Juliana; Toniazzo,Geciane; Valduga,Eunice; Di Luccio,Marco; Cansian,Rogério Luis.
The objective of this work was to evaluate the effect of different water pressures and concentrations of lactic acid on microbial counts (mesophilic bacteria, enterobacteria and Salmonella) on pig carcasses without contamination and contaminated carcasses, before and after the last shower and before being cooled. The tests were carried out using 4, 3 and 2 bar water pressure, and 2, 1 and 0% lactic acid concentration. In general, both the pressure in the shower and lactic acid had a positive effect by reducing the microbial count. The interaction between the pressure and lactic acid caused the largest reduction in carcasses surface count for mesophiles. With regard to enterobacteria on contaminated carcasses, the most important variable was the lactic acid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pig carcasses; Decontamination; Water pressure; Lactic acid; Bacteria.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600954
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Elaboração e avaliação da qualidade de macarrão isento de glúten Ciência Rural
Tomicki,Letícia; Rigo,Aline Andressa; Durigon,Angelise; Gutkoski,Luiz Carlos; Zeni,Jamile; Valduga,Eunice; Steffens,Clarice; Toniazzo,Geciane.
Com a intenção de minimizar a carência de produtos isentos de glúten e tendo em vista o crescente aumento de celíacos, a elaboração de produtos diferenciais a base de farinha de arroz e milho torna-se uma alternativa de consumo e comercialização. Assim, este trabalho teve como objetivo avaliar os efeitos da adição de farinha de arroz e milho, e emulsificante nas características de qualidade (teste de cozimento, textura instrumental - firmeza e aceitabilidade) e físico-químicas (proteínas, lipídios, carboidratos, minerais e fibra alimentar) de formulações de macarrão, mediante o emprego de metodologia de planejamento de experimentos, fixando a concentração de ovos in natura (12%, m/m) e água (20%, v/m). A formulação com 40g de farinha de milho, 60g de...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arroz; Milho; Massa; Celíacos..
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000701311
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