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| Lima,C.R.O.; Faleiro,M.B.R.; Rabelo,R.E.; Vulcani,V.A.S.; Rubini,M.R.; Torres,F.A.G.; Moura,V.M.B.D.. |
| ABSTRACT The canine transmissible venereal tumor (TVT) affects the external genitalia of dogs by the natural transplant of viable tumor cells. Thus, this research aimed to diagnose and characterize TVT morphological patterns, identify the insertion of the LINE-1 element in C-MYC gene, by means of the polymerase chain reaction (PCR), and evaluate the immunohistochemical expression of C-MYC, p53, p21 and p27 proteins. The relationship between C-MYC and p53 proteins and their interference on the expression of p21 and p27 were also studied. For that, 20 samples of naturally occurring TVT were used, subjected to cytopathological, histopathological and immunohistochemical analysis, and to molecular diagnosis of neoplasia. The increased tissue expression and the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dog; Cell; Cytology; Immunohistochemistry. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000300658 |
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| Vasconcelos,R.B.; Salles,L.P.; Oliveira e Silva,I.; Gulart,L.V.M.; Souza,D.K.; Torres,F.A.G.; Bocca,A.L.; Rosa e Silva,A.A.M.. |
| Follicle cultures reproduce in vitro the functional features observed in vivo. In a search for an ideal model, we cultured bovine antral follicle wall sections (FWS) in a serum-free defined medium (DM) known to induce 17β-estradiol (E2) production, and in a nondefined medium (NDM) containing serum. Follicles were sectioned and cultured in NDM or DM for 24 or 48 h. Morphological features were determined by light microscopy. Gene expression of steroidogenic enzymes and follicle-stimulating hormone (FSH) receptor were determined by RT-PCR; progesterone (P4) and E2 concentrations in the media were measured by radioimmunoassay. DM, but not NDM, maintained an FWS morphology in vitro that was similar to fresh tissue. DM also induced an increase in the expression... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ovary follicle wall section; FSH receptor; Gene expression; Steroidogenic enzymes; Hormone steroids; Morphological aspect. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800700 |
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