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Phylogenetic relationship of coffee leaf rust in the central jungle of Peru Acta Agron. (Palmira)
Gamarra Gamarra,Delia Palmira; Torres Suarez,Gilberto; Villar Quiñonez,Charo Milagros; McTaggart,Alistair R.; Carrasco Lozano,Emerson Clovis.
Abstract Coffee leaf rust is the main disease that causes significant losses in Coffea arabica. In Peru, this disease caused epidemics between 2008 and 2013 with production losses of 35 %. The objective was to identify H. vastatrix using a morphological and molecular approach based on a phylogenetic species concept. Coffee leaf samples with symptoms of chlorotic lesions with the presence of yellow uredospores at different severity stages of different cultivars were collected from 11 locations in the departments of Pasco and Junin during 2017-2018. DNA was purified as proposed by Cristancho and coworkers. The major subunit of ribosomal DNA was amplified with universal primers LR0R and LR5, and sequenced by Macrogen and deposited in GenBank. Sequences from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea arabica; DNA barcode; Hemileia vastatrix; Peru; Phylogeny.
Ano: 2021 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122021000200155
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