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Characterization of Listeria monocytogenes isolated from a fresh mixed sausage processing line in Pelotas-RS by PFGE BJM
von Laer,Ana Eucares; Lima,Andréia Saldanha de; Trindade,Paula dos Santos; Andriguetto,Cristiano; Destro,Maria Teresa; Silva,Wladimir Padilha da.
Listeria monocytogenes is a bacterium capable to adhere to the surfaces of equipment and utensils and subsequently form biofilms. It can to persist in the food processing environmental for extended periods of time being able to contaminate the final product. The aim of this study was to trace the contamination route of L. monocytogenes on a fresh mixed sausage processing line, from raw material to the final product. The isolates obtained were characterized by serotyping and molecular typing by pulsed-field gel electrophoresis (PFGE) using the restriction enzymes ApaI and AscI. L. monocytogenes was detected in 25% of the samples. The samples of raw material were not contaminated, however, the microorganism was detected in 21% of the environmental samples...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Listeria monocytogenes; PFGE; Serotyping; Persistent strains.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300021
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