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Detoxification of Pacific oyster Crassostrea gigas fed on diets of Skeletonema costatum with and without silt, following PSP contamination by Alexandrium minutum ArchiMer
Gueguen, Marielle; Bardouil, Michele; Baron, Regis; Lassus, Patrick; Truquet, Philippe; Massardier, Julie; Amzil, Zouher.
Contamination of shellfish by paralytic shellfish poisoning ( PSP) toxins poses an economic threat to shellfish farmers. As contaminated shellfish cannot be harvested for long periods of time, it would be very useful to develop processes to optimise and shorten their detoxification. In this study, Pacific oysters Crassostrea gigas were first experimentally contaminated over a period of 13 days with a continuous flow of toxic Alexandrium minutum cultures at concentrations ranging from 150 to 200 cell ml(-1) ( toxin content after 13 days of contamination 438 mu g STX equiv. 100 g(-1) wet weight). Then, two different detoxification treatments were tested and showed detoxification rates greater than those observed in coastal environments. The first treatment...
Tipo: Text Palavras-chave: Alexandrium minutum; Crassostrea gigas; Detoxification; Inorganic matter; Organic matter; PSP; Paralytic shellfish poisoning.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3896.pdf
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Dinoflagelles toxiques sur les cotes francaises pendant l'ete 1983.Validite du test-souris pour le controle routinier de secteurs mytilicoles contamines par la toxine du Dinophysis. ArchiMer
Marcaillou-le Baut, Claire; Le Dean, Loic; Truquet, Philippe.
Following exceptional outbreak of toxic dinoflagellate species in Vilaine Bay (summer 1983) a survey was carried out: variations in phytoplankton abundance and distribution in water and in mussel guts were followed. In the same time toxicity studies using mouse-test were performed with contaminated shellfishes, and mouse-test calibration was attempted. In spite of sampling and analysis conditions could be open to criticism, results obviously showed relation between shellfishes toxicity and occurrence of responsible species: Dinophysis acuminata.
Tipo: Text Palavras-chave: Brittany; France; ANE; Dinophysis acuminata; Red tides; Toxicity tests; Food poisoning; Biological poisons; Mussel culture.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/publication-1808.pdf
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Toxicité aiguë du tétrachloroéthylène sur la crevette des marais Palaemonetes varians ArchiMer
Miossec, Laurence; Bocquene, Gilles; Truquet, Philippe.
Studying the impact of domestic sewer discharge on marine biota we studied lethal toxicity of tetrachloroethylene on brackish water prawn (Palaemonetes varians). These experiments were carried out in a flow-through system during 96 hours : LC 50 is 2,37 mg/l. This result and aIl data, present in litterature, show the high sensitiveness of crustaceans to this chlorinated solvent.
Tipo: Text Palavras-chave: Toxicité aiguë; Tétrachloroéthylène; Crevettes; Palaemonetes varians; Flux continu; Lethal toxicity; Tetrachloroethylene; Prawn; Palemonetes varians; Flow-through system.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/00156/26706/24781.pdf
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Effect of Dilution Rate on Azadinium spinosum and Azaspiracid (AZA) Production in Pilot Scale Photobioreactors for the Harvest of AZA1 and -2 ArchiMer
Jauffrais, Thierry; Sechet, Veronique; Truquet, Philippe; Amzil, Zouher; Herrenknecht, Christine; Hess, Philipp.
Azadinium spinosum, a small dinoflagellate has recently been discovered and identified as the primary producer of azaspiracid-1 (AZA) and -2. Since AZA poisoning has been reported following consumption of contaminated shellfish it is important to have these toxins available for toxicological studies, and a sustainable production of AZAs as calibrants in monitoring programs without having to rely on natural events. In order to address this concern, continuous pilot scale cultures were carried out to evaluate the feasibility of AZA production from A. spinosum. Algae were cultured using two 100 L chemostats in series (R1 and R2), with agitation and pH control. Four different dilution rates were tested (0.15, 0.2, 0.25 and 0.3 day−1) to evaluate chemostat...
Tipo: Text Palavras-chave: Solid phase adsorption; Chemostat; Photobioreactor; Microalgae; LC-MS/MS; Tangential flow filtration.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00152/26349/24437.pdf
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Application of a digital pattern recognition system to Dinophysis acuminata and D-sacculus complexes ArchiMer
Truquet, Philippe; Lassus, Patrick; Honsell, Giorgio; Le Dean, Loic.
An image discrimination technique was developed to improve specific identification of some toxic Dinophysis cells (marine dinoflagellates involved in diarrhoeic shellfish poisoning), especially the ''acuminata'' and ''sacculus'' groups, which can be present at different ratios in natural sea-water samples collected during toxic episodes. This work was performed with image analysis software SAMBA (TITN Alcatel) using preserved cells directly observed through an inverted microscope and recorded with a video camera before further processing. All morphometric parameters used for discrimination of 7 different species or morphotypes of Dinophysis were tested with discriminant analysis. This study indicates that Dinophysis sp. and D. pavillardi seem well...
Tipo: Text Palavras-chave: Dinophysis sp.; Analyse d'image; Taxonomie; Phytoplancton; Dinophysis sp; Image analysis; Taxonomy; Phytoplankton.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00188/29890/28337.pdf
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Spirolide uptake and detoxification by Crassostrea gigas exposed to the toxic dinoflagellate Alexandrium ostenfeldii ArchiMer
Medhioub, Walid; Lassus, Patrick; Truquet, Philippe; Bardouil, Michele; Amzil, Zouher; Sechet, Veronique; Sibat, Manoella; Soudant, Philippe.
Oysters (Crassostrea gigas) were exposed 4 days to cultures of the toxic dinoflagellate Alexandrium ostenfeldii (strain CCMP1773) that produces spirolides belonging to fast acting toxins (FAT) and let depurate for 7 days. During depuration, oysters were either fed the non-toxic algae Isochrysis galbana Tahitian clone (T. Iso) or starved. The objectives of this experiment were to evaluate (i) spirolide uptake and depuration by oysters (ii) spirolide effects on oysters and (iii) oyster recovery according to food supply during depuration. A. ostenfeldii cells were filtered and ingested by oysters while faeces contained numerous intact cells of the toxic diet. This suggested that ingested cells were not totally digested by oysters. Contents of spirolides (SPX)...
Tipo: Text Palavras-chave: Spirolides; Alexandrium ostenfeldii; Contamination; Depuration; Crassostrea gigas; Inflammatory responses.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00098/20953/18740.pdf
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Influence de la température et de la salinité sur les perturbations phytoplanctoniques observées dans le Mor Bras en 1983 et 1984 ArchiMer
Truquet, Isabelle; Lassus, Patrick; Truquet, Philippe; Maggi, Pierre.
L'équilibre des écosystèmes phytoplanctoniques sur le littoral sud breton a été modifié à deux reprises, en 1983 et 1984, et selon une chronologie apparemment identique, à savoir: des eaux colorées (plus de 106 cellules par litre) à diatomées apparaissant soit en mars-avril (Skeletonema costatum) soit en juin (Nitzschia, Rhizoselenia et Chaetoceros) et suivies dans le second cas par des concentrations inhabituelles de certaines espèces de dinoflagellés.
Tipo: Text
Ano: 1985 URL: http://archimer.ifremer.fr/doc/00139/25016/23120.pdf
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Influence de chocs thermiques sur la croissance d'une diatomée : phaeodactylum tricornutum Bohlin - Annexes ArchiMer
Schaeffer, F.; Truquet, Philippe; Richard, P.; Polleau, J.r..
Tipo: Text
Ano: 1975 URL: http://archimer.ifremer.fr/doc/00046/15757/13159.pdf
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Influence de la Chloration sur les Premiers Stades Larvaires de Palaemon serratus (Pennant). ArchiMer
Lassus, Patrick; Maggi, Pierre; Le Baut, Claire; Bardouil, Michèle; Bocquene, Gilles; Le Dean, Loïc; Truquet, Philippe.
In order to estimate the effects of chlorine used in cooling systems of electric power plants on zooplankton, some experiments have been realized with the four first larval stages of the prawn (P. serratus Pennant). Chlorine injection was performed by constant adding at 16 and 20 degree C with times exposures of 5, 15 and 25 minutes. If there is no detectable variations in sensitivity of different stages, toxic effects of oxydants issued from chlorination can be observed (for both the two acclimatation temperatures) at 15 minutes exposure with a LD 50 : 32 mg/l of chlorine.
Tipo: Text Palavras-chave: Palaemon serratus; Pollution effects; Larval development; Chlorine; Toxicity.
Ano: 1981 URL: http://archimer.ifremer.fr/doc/1981/publication-1886.pdf
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Paralytic shellfish poison accumulation yields and feeding time activity in the Pacific oyster (Crassostrea gigas) and king scallop (Pecten maximus) ArchiMer
Bougrier, Serge; Lassus, Patrick; Bardouil, Michele; Masselin, Pierre; Truquet, Philippe.
Pacific oysters and king scallops placed individually in a recirculating flume were fed for 2 weeks with a constant concentration (120 cell ml(-1)) of a toxic strain of Alexandrium minutum. Fluorescence at the outlet of each experimental unit was measured continuously, and biodeposits were recovered twice daily to evaluate feeding time activity (FTA) and rates of organic filtration (OFR), ingestion (OIR) and organic absorption (OAR). Ion-pairing high performance liquid chromatography (IP-HPLC) was performed concurrently to quantify paralytic phycotoxins both (i) individually in shellfish at the end of the contamination period and (ii) in A. minutum cultures to estimate cellular toxin concentration. These data allowed comparison of the actual tissue toxin...
Tipo: Text Palavras-chave: Alexandrium minutum; Crassostrea gigas; Feeding activity; Paralytic shellfish poisoning.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-576.pdf
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Improving detoxification Efficiency of PSP-contaminated oysters (Crassostrea gigas Thunberg) ArchiMer
Lassus, Patrick; Bardouil, Michele; Baron, Regis; Berard, Jean-baptiste; Masselin, Pierre; Truquet, Philippe; Pitrat, Jp.
Shellfish production, and production of marine bivalve molluscs in particular, is the main form of marine aquaculture in the European Union, with a production estimated at 1,200,000 tons in 1999 (Food and Agricultural Organization). Moreover, aquaculture production is rapidly expanding throughout Europe and is becoming a major economic resource in developing areas such as Northern Greece, Ireland, and northwestern Spain. These shellfish, mainly mussels, oysters, and clams, are produced in a natural environment. Due to variations in their environment, these otherwise edible species may however become unsafe for human consumption. Potential problems are amplified due to the nature of shellfish themselves: as they are filter feeders, they consume large...
Tipo: Text Palavras-chave: Detoxification; PSP toxin; Toxic algae; Bioaccumulation; Contaminant; Clam; Mussel; Oyster; Shellfish culture.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-2271.pdf
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Evaluation du potentiel nutritif des eaux de la baie de Vilaine pendant l'été 1987 ArchiMer
Le Dean, Loic; Truquet, Philippe; Lassus, Patrick.
The growth potential study of waters of the Vilaine bay was realized from march to september 1987 and for three sampling stations. The growth of depleted test cells of Skeletonema costatum was estimated and compared from existing nutrients pools for aIl water samples. As a first result it appears that inorganic nitrogen could stimulate the growth during spring while no clear relationship is observed between the algal growth and other hydrological parameters during spring or summer periods. Nevertheless these first results need further corroboration.
Tipo: Text Palavras-chave: Potentiel nutritif; Baie de Vilaine; Skeletonema costatum; Test biologique; Growth potential; Vilaine Bay; S. costatum; Bioassay.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/00139/25037/23146.pdf
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Gut passage times in two bivalve molluscs fed toxic microalgae: Alexandrium minutum, A-catenella and Pseudo-nitzschia calliantha ArchiMer
Gueguen, Marielle; Lassus, Patrick; Laabir, Mohamed; Bardouil, Michele; Baron, Regis; Sechet, Veronique; Truquet, Philippe; Amzil, Zouher; Barille, Laurent.
The occurrence of new phytoplankton species in a coastal area may be explained by the import of shellfish containing whole live algal cells in their digestive tracts. Indeed, shellfish containing toxic algal cells can induce both primary contaminations in safe areas ( initially free from toxic microalgae), and secondary contaminations of other shellfish growing in the same area via the faeces of the imported animals. To mitigate this problem, shellfish need to be placed in a separate holding tank and their intestinal content purged. For a deeper understanding of the risks associated with transferring contaminated shellfish, oysters ( Crassostrea gigas) and mussels ( Mytilus edulis) were purposely fed either Alexandrium minutum or A. catenella (...
Tipo: Text Palavras-chave: Risk assessment; Toxic phytoplankton; Uptake; Gut passage; Mytilus edulis; Crassostrea gigas.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3897.pdf
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Influence de chocs thermiques sur la croissance d'un dinoglagelle responsable d'eaux rouges: Scrippsiella faeroense (Paulsen) BALECH et SOAREZ ArchiMer
Lassus, Patrick; Le Baut, Claire; Truquet, Philippe; Bardouil, Michèle; Le Dean, Loïc; Bocquene, Gilles.
The growing development of thermonuclear electric plants on coastal areas drove the I.S.T.P.M. to carry out a number of impact studies. Previous works on the impact of the transit in the condensers on the phytoplankton have been published by the Pollution Biological Effects laboratory (Maggi el al., 1979 and 1980; Lassus and Maggi, 1980). Our results on the chlorophycea Dunaliella terliolecla demonstrated that thermic elevations from low temperatures stimulated its cellular development. Therefore, it seemed interesting to measure the effects of these thermic shocks on dinophyceae (or dinoflagellates), components, with diatoms, of the coastal phytoplankton, and main agents of the summer phenomena known as "red waters".
Tipo: Text
Ano: 1982 URL: http://archimer.ifremer.fr/doc/1982/publication-6674.pdf
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Distribution verticale de Dinophysis spp au cours d'un cycle de 48 heures dans le port d'Antifer (Normandie) ArchiMer
Lassus, Patrick; Maggi, Pierre; Proniewski, F.; Truquet, Philippe; Nizard, G..
Time-related Dinophysis spp vertical profiles have been studyed during a 48 hours cycle in a submerged cylindric tank. A coïncidental Gymnodinium sp. (Scrippsiella trochoidea and Prorocentrum triestinum as companion species) red tide was believed to have strong effect on Dinophysis distribution in the upper layer during the tirst 24 hours cycle of the period of investigation (August 30-31, 1991). Between 24 and 48 hours, the sharp decrease of Dinophysis densities as well as the graduai decline observed in the red tide and in the vertical thermal gradient have resulted in a migration of dominant species : D. sacculus and D. cf. acuminata toward subsuperficiallevels (5 to 7 m.d) with a higher range in cells vertical movements. This behavior is very similar...
Tipo: Text
Ano: 1991 URL: http://archimer.ifremer.fr/doc/00138/24948/23041.pdf
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Paralytic Shellfish Poisoning on French Mediterranean coast in the autumn of 1998:Alexandrium tamarense as a causative agent ArchiMer
Masselin, Pierre; Amzil, Zouher; Carreras, Antoni; Chiantella, Claude; Le Bec, Claude; Nezan, Elisabeth; Truquet, Philippe.
Tipo: Text
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00017/12871/9821.pdf
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Effect of Azadinium spinosum on the feeding behaviour and azaspiracid accumulation of Mytilus edulis ArchiMer
Jauffrais, Thierry; Contreras, Andrea; Herrenknecht, Christine; Truquet, Philippe; Sechet, Veronique; Tillmann, Urban; Hess, Philipp.
Azadinium spinosum, a small toxic dinoflagellate, was recently isolated and identified as a primary producer of azaspiracid toxins (AZAs). Previous experiments related to AZA accumulation in blue mussels upon direct feeding with A. spinosum revealed increased mussel mortality and had negative effects on the thickness of the digestive gland tubules. Therefore we conducted follow up experiments in order to study effects of A. spinosum on mussel feeding behaviour. Individual assessment of mussel feeding time activity (FTA), clearance rate (CR), filtration rate (TFR), absorption rate (AR), faeces and pseudofaeces production were carried out on mussel fed either toxic (A. spinosum) or non-toxic (Isochrisis aff. galbana (T-Iso)) diets. Furthermore, AZA...
Tipo: Text Palavras-chave: Bivalve molluscs; Mussel; Ecophysiology; AZA biotransformation; AZA accumulation; Trophic transfer; Dinoflagellate; Azaspiracid.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00090/20174/17832.pdf
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Detoxification enhancement in the gymnodimine-contaminated grooved carpet shell, Ruditapes decussatus (Linne) ArchiMer
Medhioub, Walid; Gueguen, Marielle; Lassus, Patrick; Bardouil, Michele; Truquet, Philippe; Sibat, Manoella; Nejib, Medhioub; Philippe, Soudant; Mejdeddine, Kraiem; Amzil, Zouher.
In the Gulf of Gabes (Tunisia, Eastern Mediterranean sea), the grooved carpet shell Ruditapes decussatus has been seen to contain persistent levels of gymnodimine (GYM) for several years. The present experimental work represents the first attempt to assess detoxification kinetics of fast-acting toxins (FAT) in marine molluscs fed specific diets of non-toxic algae (Isochrysis galbana). To find an optimal detoxification method, two experiments were performed in which clams were first fed the toxic dinoflagellate Karenia selliformis to artificially contaminate them with GYM, thus simulating the effect of natural toxic episodes. As a second step, the same clams were fed a non-toxic algae, I. galbana. to speed up the detoxification process. Changes in toxin...
Tipo: Text Palavras-chave: Ruditapes decussates; Grooved carpet shell; Karenia selliformis; Gymnodimine; Toxicity; Detoxification.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/2010/publication-7488.pdf
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Modélisation de la contamination des bivalves par les toxines d'Alexandrium spp. Rapport final ArchiMer
Lassus, Patrick; Amzil, Zouher; Bardouil, Michele; Baron, Regis; Bohec, Madeleine; Bougrier, Serge; Garen, Pierre; Laabir, Mohamed; Leguay, Didier; Masselin, Pierre; Truquet, Philippe; Sechet, Veronique; Bablon, Clotilde; Berard, Jean-baptiste; Carre, Camille; Cheignon, Valerie; Lecamp, Sylvain; Mabit, Marie.
L'objectif général de l'étude est de simuler les épisodes de contamination de certains bivalves (huîtres et moules) par les toxines d'Alexandrium minutum et A. calenella à partir d'un module expérimental adapté, et en vue de construire un modèle de contamination applicable à ces espèces. Ce modèle sera ensuite susceptible d'être couplé aux modèles biologiques locaux (estuaire de Penzé et étang de Thau) de déterminisme des blooms, ainsi qu'aux modèles hydrodynamiques développés par différentes équipes (Ifremer, Université de Montpellier). La première année (2001-2002) était consacrée à la simulation des contaminations en estuaire de Penzé (Alexandrium minulum) et au développement de cultures en masse d'A. calenella de Thau en bioréacteurs.
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Ano: 2002 URL: http://archimer.ifremer.fr/doc/00156/26711/24786.pdf
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Modélisation de la contamination des huîtres par les toxines d'Alexandrium minutum - Rapport final (2ème année) ArchiMer
Lassus, Patrick; Bardouil, Michele; Baron, Regis; Garen, Pierre; Laabir, Mohamed; Leguay, Didier; Masselin, Pierre; Truquet, Philippe; Joret, Camille.
L'objectif de l'étude est de simuler les épisodes de contamination des huîtres par les toxines d'Alexandrium minufum à partir d'un module expérimental adapté, et en vue de construire un modèle de contamination applicable à cette espèce. Ce modèle sera ensuite susceptible d'être couplé au modèle biologique local (estuaire de Penzé) de déterminisme des blooms, ainsi qu'aux modèles hydrodynamiques développés par différentes équipes (Ifremer).
Tipo: Text
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00156/26712/24787.pdf
Registros recuperados: 48
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