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Catalase activity of different Candida species after exposition to specific antiserum BJM
Miyasaka,Natália R.S.; Unterkircher,Carmelinda S.; Shimizu,Mario T..
Antisera were developed in rabbits after challenge with intracellular antigens of Candida albicans, C. tropicalis and C. parapsilosis. Microorganism catalase has been correlated with virulence, resistance to drugs and immunogenicity. The intracellular catalase is consistently present in strains of Candida and in this paper, the enzyme activity was analysed by PAGE after exposition to antisera. The catalases of C. albicans, C. parapsilosis and C. tropicalis were immunogenic and differed in their binding to specific antibodies raised in rabbits. Tests of cross-reactivity between different Candida species showed that when antiserum from C. albicans immunized rabbit was incubated with intracellular extracts of these three Candida species, the catalases...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candidiasis; Catalase; Candida; Antigen; Diagnosis.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100009
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Natural antibodies in paracoccidioidomycosis BJM
Unterkircher,Carmelinda S.; Leão,Mariella V.P.; Blotta,Maria Heloisa S.L.; Camargo,Zoilo Pires de.
Recent attention has been focused on the natural antibodies as a component of natural immunity and as integral part of the idiotypic network. However, their functional role in different infections has rarely been studied. This work was undertaken to investigate the presence of natural antibodies in paracoccidioidomycosis (PCM). In addition, we analyzed anti-P. brasiliensis antibodies and their distribution in IgG subclasses in order to acquire better knowledge about the humoral immune response in this mycosis. Our findings show that the natural antibody response is not very much increased in PCM when compared with other parasite infections and this response is restricted to a few specificities, suggesting that P. brasiliensis moderately triggers CD5+ B...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Natural antibodies; Paracoccidioides brasiliensis; Paracoccidioidomycosis.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100009
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