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| Labry, Claire; Urvoy, Marion. |
| Alkaline phosphatases are the main enzymes required by microorganisms to hydrolyse organic phosphorus into available phosphate in aquatic environments. The investigations of alkaline phosphatase activity (APA) usually generate numerous samples (size fractionation, Michaelis-Menten kinetics). Therefore, convenient and reliable preservation of incubated samples for a deferred analysis would be very useful when measurements cannot be performed right away. The APA of marine pond waters was measured using 4-Methylumbelliferyl phosphate (MUF-P) as the fluorogenic substrate modelling natural organic phosphorus compounds. Where typical inhibitors of other enzymatic activities, such as 1% sodium dodecyl sulfate, mercuric chloride, or buffered solutions of ammonium... |
| Tipo: Text |
Palavras-chave: Alkaline phosphatase activity; Formaldehyde; Inhibition; Enzymes; Aquatic studies; Deferred measurements. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00656/76787/78211.pdf |
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| Urvoy, Marion; Labry, Claire; Delmas, Daniel; Creac'H, Layla; L Helguen, Stephane. |
| As microbial enzymatic activities initiate the mineralization of organic matter through the microbial loop, it is important to correctly measure those activities and be able to perform inter-study comparisons. Enzymatic activity assays are typically carried out using fluorogenic substrate analogs, such as 4-Methylumbelliferone and 7-Amino-4-methylcoumarin linked to sugar monomers, phosphate group or amino acids. However, methodological divergences can be found in aquatic science literature, potentially leading to misestimated activities. To highlight some of those methodological key points, we first addressed the potential occurrence of an Inner Filter Effect (IFE), a fluorometric artifact that affects the relationship between fluorophore concentration and... |
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| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00653/76524/77614.pdf |
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