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Paula,Daniella A. J.; Machado,Mônica R. F.; Murgas,Luis D. S.; Rabelo,Denílson; Zangerônimo,Márcio G.; V. R. Neto,Rafael; Andrade,Estefânia S.; Andrade,Eliane A.. |
This paper investigated the effect of cryoprotectant substances on Prochilodus lineatus embryos in an experimental incubator. The prospective study applied combinations of polyvinyl alcohol, hydroxyethyl cellulose, gelatin and fetal bovine serum with dimethyl sulfoxide and ethylene glycol in a new experimental incubator. The morphology of embryos, larval viability and the efficiency of experimental incubators in maintaining the quality of embryos were evaluated. This study demonstrates the efficient association between hydroxyethylcellulose and dimethyl sulfoxide as greater viability (p<0.05) was found for embryos (72.9 ± 23.9%). It should also be noted the permeation of cryoprotectants in embryos through the changes found in chorion diameter, embryo... |
Tipo: Info:eu-repo/semantics/article |
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Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000400835 |
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