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Complete sequence of the genome of the human isolate of Andes virus CHI-7913: comparative sequence and protein structure analysis Biol. Res.
TISCHLER,NICOLE D; FERNÁNDEZ,JORGE; MÜLLER,ILSE; MARTÍNEZ,RODRIGO; GALENO,HÉCTOR; VILLAGRA,ELIECER; MORA,JUDITH; RAMÍREZ,EUGENIO; ROSEMBLATT,MARIO; VALENZUELA,PABLO D.T..
We report here the complete genomic sequence of the Chilean human isolate of Andes virus CHI-7913. The S, M, and L genome segment sequences of this isolate are 1,802, 3,641 and 6,466 bases in length, with an overall GC content of 38.7%. These genome segments code for a nucleocapsid protein of 428 amino acids, a glycoprotein precursor protein of 1,138 amino acids and a RNA-dependent RNA polymerase of 2,152 amino acids. In addition, the genome also has other ORFs coding for putative proteins of 34 to 103 amino acids. The encoded proteins have greater than 98% overall similarity with the proteins of Andes virus isolates AH-1 and Chile R123. Among other sequenced Hantavirus, CHI-7913 is more closely related to Sin Nombre virus, with an overall protein...
Tipo: Journal article Palavras-chave: Hantavirus; Genome sequence; RNA polymerase; Human isolate.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200010
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Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis Biol. Res.
WILHELM,VIVIAN; SOZA,CRISTIÁN; MARTÍNEZ,RODRIGO; ROSEMBLATT,MARIO; BURZIO,LUIS O; VALENZUELA,PABLO D.T..
We have isolated and sequenced the genes encoding the heat shock proteins 60 (Hsp60) and 70 (Hsp70) of the salmon pathogen Piscirickettsia salmonis. The sequence analysis revealed the expected two open reading frames that encode proteins with calculated molecular weights of 60,060 and 70,400. The proteins exhibit a 70-80% homology with other known prokaryotic Hsp60 and Hsp70 sequences. The coding regions have been expressed in E. coli as thioredoxin fusion proteins. Both recombinant proteins were shown to elicit a humoral response when injected intraperitoneally in Atlantic salmon and also conferred protection to fish challenged with P. salmonis. The present data will facilitate further studies on the involvement of heat shock proteins in protective...
Tipo: Journal article Palavras-chave: Piscirickettsia salmonis; Hsp60; Hsp70; GroEL; DnaK; Immune response.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100009
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The complete sequence of the mitochondrial genome of the Chinook salmon, Oncorhynchus tshawytscha Biol. Res.
WILHELM,VIVIAN; VILLEGAS,JAIME; MIQUEL,ÁLVARO; ENGEL,ESTEBAN; BERNALES,SEBASTIÁN; VALENZUELA,PABLO D.T.; BURZIO,LUIS O..
The complete sequence of the mitochondrial genome of Chinook salmon, Oncorhynchus tshawytscha, has been determined. The circular genome consisting of 16,644 base pairs encodes thirteen proteins, the 12S and 16S ribosomal RNAs, and 22 transfer RNAs. These genes are ordered in the same way as most other vertebrates. The nucleotide and amino acid sequences of the ribosomal RNAs and the thirteen protein-coding genes were compared with those of other salmonids such as Oncorhynchus mykiss, Salmo salar, Salvelinus fontinalis, Salvelinus alpinus and Coregonus lavaretus. The sequence features of the control region (D-loop), the origin of L-strand replication and a putative peptide codified by the 16S mitochondrial RNA are described and discussed.
Tipo: Journal article Palavras-chave: Salmonids; Mitochondria; Genome; Peptides; 16S rRNA.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200012
Registros recuperados: 3
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