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| MÜLLER,ILSE; MEDINA-SELBY,ANGELICA; PALACIOS,JOSE LUIS; MARTINEZ,PATRICIO; OPAZO,PATRICIO; BRUCE,ELSA; MANCILLA,MARTA; VALENZUELA,PABLO; YUDELEVICH,ARTURO; VENEGAS,ALEJANDRO. |
| We have cloned and sequenced ten Helicobacter pylori genes from a Chilean strain (CH-CTX1) including: a cytotoxin VacA fragment, a CagA fragment (A17), a species-specific protein (TsaA), urease subunits (UreA, UreB), a flagellin subunit (FlaB), heat shock proteins (HspA and HspB), adhesin (HpaA) and a lipoprotein (Lpp20). We compared their deduced amino acid sequences with the corresponding sequences from three unrelated H. pylori strains, including fully sequenced strains 26695(UK) and J99(USA), and found that eight of them (UreA, UreB, FlaB, HspA, HspB, Lpp20, TsaA and HpaA) presented more than 97.3% identity. In contrast, VacA partial sequence showed lower identity values (93.2 - 94.9%). Moreover, we found major differences in the A17 region respect to... |
| Tipo: Journal article |
Palavras-chave: Chilean H. pylori strain; PCR-cloned genes; HpaA; VacA; CagA sequences. |
| Ano: 2002 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100010 |
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| TORO,ANDRÉS; ARREDONDO,CRISTIAN; CORDOVA,GONZALO; ARAYA,CLAUDIA; PALACIOS,JOSÉ L; VENEGAS,ALEJANDRO; MORITA,MASASHI; IMANAKA,TSUNEO; SANTOS,MANUEL J. |
| Peroxisomes are thought to be formed by division of pre-existing peroxisomes after the import of newly synthesized proteins. However, it has been recently suggested that the endoplasmic reticulum (ER) provides an alternative de novo mechanism for peroxisome biogenesis in some cells. To test a possible role of the ER-Golgi transit in peroxisome biogenesis in mammalian cells, we evaluated the biogenesis of three peroxisomal membrane proteins (PMPs): ALDRP (adrenoleukodystrophy related protein), PMP70 and Pex3p in CHO cells. We constructed chimeric genes encoding these PMPs and green fluorescent protein (GFP), and transiently transfected them to wild type and mutant CHO cells, in which normal peroxisomes were replaced by peroxisomal membrane ghosts. The... |
| Tipo: Journal article |
Palavras-chave: Adrenoleukodystrophy; ALDRP; Endoplasmic reticulum; Peroxisome biogenesis; Pex3p and PMP70. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200014 |
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| RIOS,MARIANA; VENEGAS,ALEJANDRO; CROXATTO,HORACIO B. |
| Intra-oviductal administration of RNA obtained from oviducts of estradiol-treated rats resulted in accelerated egg transport (<A HREF="#rios97">Ríos et al., 1997</A>). It is probable that estradiol-induced messenger RNA (mRNA) entered oviductal cells and was translated into the proteins involved in accelerated egg transport. In order to test this interpretation we deposited in vivo 50 µg of pure ß-galactosidase (ß-gal) mRNA, 50 µg of pure DNA from the reporter gene ß-gal under SV40 promoter or the vehicle (control oviducts) into the oviductal lumen of rats. Twenty four hours later the ß-gal activity was assayed in oviductal tissue homogenates using o-nitrophenyl-ß-D-galactopyranoside as a substrate. The administration of ß-gal mRNA and pSVBgal... |
| Tipo: Journal article |
Palavras-chave: SS-galactosidase; Gene transfer; Oviduct. |
| Ano: 2002 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300007 |
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