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| LÓPEZ,MAURICIO; FIGUEROA,JAIME; KAUSEL,GUDRUN; VERA,MARÍA INÉS; KRAUSKOPF,MANUEL. |
| We isolated and cloned a carp somatolactin SL DNA fragment, of which 78% of the nucleotides were identical to the corresponding salmon SL sequence. The results obtained upon Northern blot hybridization of carp pituitary RNA allowed the identification of two transcripts as described for other fish. When the content of SL transcripts in pituitary sections from summer- and winter- acclimatized carp was quantified by in situ hybridization assays, we found no significant differences between the two seasons. In salmonids, plasma SL reaches higher levels in summer than in winter in synchrony with the water temperature cycle; in the eurythermal carp, however, the complex adaptive responses imposed by seasonal environmental changes do not seem to include the... |
| Tipo: Journal article |
Palavras-chave: Acclimatization; In situ hybridization; Somatolactin expression; Pituitary; Carp; Fish. |
| Ano: 2001 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100010 |
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| VERA,MARÍA INÉS; MOLINA,ALFREDO; PINTO,RODRIGO; REYES,MAURICIO; ÁLVAREZ,MARCO; KRAUSKOPF,ERWIN; QUEZADA,CLAUDIA; TORRES,JORGE; KRAUSKOPF,MANUEL. |
| The seasonal adaptation of the teleost Cyprinus carpio to the cyclical changes of its habitat demands physiological compensatory responses. The process involves profound nucleolar adjustments and remarkable changes in rRNA synthesis, which affects ribosomal biosynthesis. In this context, we have demonstrated that the synthesis of several proteins involved in ribosomal biogenesis as protein kinase CK2, ribosomal protein L41 and nucleolin, as well as U3 snoRNP, are differentially regulated in summer-acclimatized carp compared to the cold-season adapted fish. To understand the mechanisms involved in the seasonal regulation of rRNA gene transcription, we have been studying the carp rDNA cistron structure. Because the cis-elements that regulate the expression... |
| Tipo: Journal article |
Palavras-chave: Ribosomal gene; RRNA; RDNA; Fish; Carp. |
| Ano: 2003 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200014 |
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