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| Valadares,Marize Campos; Pereira,Enir Raquel Tavares; Benfica,Polyana Lopes; Paula,José Realino. |
| In the present study, the ability of Punica granatum ethanolic leaf extract (PGL) and Punica granatum ethanolic fruit extract (PGF) to induce mutagenicity or to modulate the genotoxic effects induced by the alkylating agent cyclophosphamide (CP) was evaluated. Swiss male mice were treated by gavage for 10 days with PGL or PGF (12.5, 25, 50, and 75 mg/kg/day) prior to exposure to CP (i.p. 200 mg/kg), 24 h after the end of the treatment. Initial observations revealed that normal mice treated with both extracts (12.5, 25, 50, and 75 mg/kg/day) showed a similar micronucleated polychromatic erythrocyte (MNPCE) frequency to that of the control group. Investigation of the protective effect of PGL and PGF based on data analysis revealed that, irrespective of dose... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Punica granatum/mutagenic effects; Micronucleic; Genotoxicity; Cyclophosphamide. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000100014 |
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| Cortez,Alane Pereira; Menezes,Elizabeth Gomes Paulino; Benfica,Polyana Lopes; Santos,Alexandre Pereira dos; Cleres,Larissa Moreira; Ribeiro,Higor de Oliveira; Lima,Eliana Martins; Kato,Massuo Jorge; Valadares,Marize Campos. |
| Abstract In this study, the potential antileukemic activity of grandisin, a lignan extracted from Piper solmsianum, was evaluated against the leukemic line K562. The cytotoxicity of grandisin (0.018 to 2.365 µM) was evaluated in K562 and normal peripheral blood lymphocytes by Trypan Blue Exclusion and MTT methods after 48h exposure to the drug. In both methods, cellular viability was concentration-dependent and the IC50 values were lower than 0.85µM. Analysis of K562 cells after treatment with grandisin showed that the cell cycle was arrested in the G1 phase with a 12.31% increase, while both S and G2 phases decreased. Morphological studies conducted after the exposure of K562 to grandisin revealed changes consistent with the apoptosis process, which was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Grandisin/antileukemic activities; Leukemic; K562; Apoptosis.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100610 |
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| Ávila,Renato Ivan de; Guerra,Meirielle Teixeira; Borges,Kamilla de Almeida Souza; Vieira,Marcelo de Sousa; Oliveira Júnior,Luiz Marcos de; Furtado,Humberto; Mota,Mariana Flavia da; Arruda,Andréa Fernandes; Valadares,Marize Campos. |
| This study investigated the chemoprotective effects of Punica granatum L. (Punicaceae) fruits alcoholic extract (PGE) on mice exposed to hexavalent chromium [Cr(VI)]. Animals were pretreated with PGE (25, 50 or 75 mg/kg/day) for 10 days and subsequently exposed to a sub-lethal dose of Cr(VI) (30 mg/kg). The frequency of micronucleated polychromatic erythrocytes in the bone marrow was investigated and the Cr(VI) levels were measured in the kidneys, liver and plasm. For the survival analysis, mice were previously treated with PGE for 10 days and exposed to a single lethal dose of Cr(VI) (50 mg/kg). Exposure to a sub-lethal dose of Cr(VI) induced a significant increase in the frequency of micronucleated cells. However, the prophylactic treatment with PGE led... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chemoprevention; Hexavalent chromium/induced genotoxicity; Oxidative damage; Pomegranate/chemoprotective effects; Punica granatum/chemoprotective effects; Punica granatum/pharmacognosy; Punicaceae/chemoprotective effects; Punicaceae/pharmacognosy. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400008 |
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| Ávila,Renato Ivan de; Ferreira,Camila Carvalho; Alvarenga,Cátia Belo Mattos; Vieira,Marcelo de Sousa; Cortez,Alane Pereira; Batista,Aline Carvalho; Costa,Elson Alves; Valadares,Marize Campos. |
| This study evaluated the safety of Lafoensia pacari A. St.-Hil. (Lythraceae) stem bark hydroalcoholic extract (LPE) through acute and subchronic toxicological assessments in mice. In the acute toxicity evaluation, a single 2000 mg/kg oral dose of LPE was administered to mice and clinical observations were conducted for 14 days. For subchronic toxicity, LPE doses (6.25-1000 mg/kg) were administered orally for 28 days and biochemical, hematological, histopathological analyses and renal and liver expression of Ki-67 were carried out. The acute oral toxicity evaluation of LPE showed no toxicity in mice and it was was classified as category 5 (LD50>2000-5000 mg/kg). In a repeated dose 28-day toxicity study, LPE (100-1000 mg/kg) led to an increase in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mangava-brava; Lafoensia pacari/evaluation; Medicinal plant; Natural product. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100595 |
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