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Sedimentological imprint on subseafloor microbial communities in Western Mediterranean Sea Quaternary sediments ArchiMer
Ciobanu, Maria Cristina; Rabineau, Marina; Droz, Laurence; Revillon, Sidonie; Ghiglione, J. -f.; Dennielou, Bernard; Jorry, Stephan; Kallmeyer, J.; Etoubleau, Joel; Pignet, Patricia; Crassous, Philippe; Vandenabeele-trambouze, Odile; Laugier, J.; Guegan, Matthieu; Godfroy, Anne; Alain, Karine.
An interdisciplinary study was conducted to evaluate the relationship between geological and paleoenvironmental parameters and the bacterial and archaeal community structure of two contrasting subseafloor sites in the Western Mediterranean Sea (Ligurian Sea and Gulf of Lion). Both depositional environments in this area are well-documented from paleoclimatic and paleooceanographic point of views. Available data sets allowed us to calibrate the investigated cores with reference and dated cores previously collected in the same area, and notably correlated to Quaternary climate variations. DNA-based fingerprints showed that the archaeal diversity was composed by one group, Miscellaneous Crenarchaeotic Group (MCG), within the Gulf of Lion sediments and of nine...
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Ano: 2012 URL: http://archimer.ifremer.fr/doc/00090/20171/17826.pdf
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Physiological features of Halomonas lionensis sp. nov., a novel bacterium isolated from a Mediterranean Sea sediment ArchiMer
Gaboyer, Frederic; Vandenabeele-trambouze, Odile; Cao, Junwei; Ciobanu, Maria-cristina; Jebbar, Mohamed; Le Romancer, Marc; Alain, Karine.
A novel halophilic bacterium, strain RHS90T, was isolated from marine sediments from the Gulf of Lions, in the Mediterranean Sea. Its metabolic and physiological characteristics were examined under various cultural conditions, including exposure to stressful ones (oligotrophy, high pressure and high concentrations of metals). Based on phylogenetic analysis of the 16S rRNA gene, the strain was found to belong to the genus Halomonas in the class Gammaproteobacteria. Its closest relatives are H. axialensis and H. meridiana (98% similarity). DNA-DNA hybridizations indicated that the novel isolate is genotypically distinct from these species. The DNA G+C content of the strain is 54.4 mol%. The main fatty acids (C18:1ω7c, 2-OH iso-C15:0, C16:0 and/or C19:0 cyclo...
Tipo: Text Palavras-chave: Halomonas; Taxonomy; Environmental adaptation; Metal tolerance; Poly-beta-hydroxyalkanoate; Compatible.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00201/31253/29658.pdf
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Microorganisms persist at record depths in the subseafloor of the Canterbury Basin ArchiMer
Ciobanu, Maria Cristina; Burgaud, Gaetan; Dufresne, Alexis; Breuker, Anja; Redou, Vanessa; Ben Maamar, Sarah; Gaboyer, Frederic; Vandenabeele-trambouze, Odile; Lipp, Julius Sebastian; Schippers, Axel; Vandenkoornhuyse, Philippe; Barbier, Georges; Jebbar, Mohamed; Godfroy, Anne; Alain, Karine.
The subsurface realm is colonized by microbial communities to depths of >1000 meters below the seafloor (m.b.sf.), but little is known about overall diversity and microbial distribution patterns at the most profound depths. Here we show that not only Bacteria and Archaea but also Eukarya occur at record depths in the subseafloor of the Canterbury Basin. Shifts in microbial community composition along a core of nearly 2 km reflect vertical taxa zonation influenced by sediment depth. Representatives of some microbial taxa were also cultivated using methods mimicking in situ conditions. These results suggest that diverse microorganisms persist down to 1922 m.b.sf. in the seafloor of the Canterbury Basin and extend the previously known depth limits of...
Tipo: Text Palavras-chave: Deep biosphere; Subsurface life; Eukaryote; Record depth.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00201/31243/29895.pdf
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Photochemistry on the Space Station-Antibody Resistance to Space Conditions after Exposure Outside the International Space Station ArchiMer
Coussot, Gaelle; Le Postollec, Aurelie; Faye, Clement; Baque, Mickael; Vandenabeele-trambouze, Odile; Incerti, Sebastien; Vigier, Flavie; Chaput, Didier; Cottin, Herve; Przybyla, Bartos; Berger, Thomas; Dobrijevic, Michel.
Antibody-based analytical instruments are under development to detect signatures of life on planetary bodies. Antibodies are molecular recognition reagents able to detect their target at sub-nanomolar concentrations, with high affinity and specificity. Studying antibody binding performances under space conditions is mandatory to convince space agencies of the adequacy of this promising tool for planetary exploration. To complement previous ground-based experiments on antibody resistance to simulated irradiation, we evaluate in this paper the effects of antibody exposure to real space conditions during the EXPOSE-R2 mission outside the International Space Station. The absorbed dose of ionizing radiation recorded during the 588 days of this mission (220 mGy)...
Tipo: Text Palavras-chave: Astrobiology; Cosmic rays; Biochip; Antibody; Planetary exploration.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00601/71305/69721.pdf
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