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	Provedor de dados BJMBR (4) Autor Varanda,W.A. (4) Aguiar,J.F. (1) Baptista,V. (1) Bustamante,J.O. (1) Cedraz-Mercez,P.L. (1) Dagostin,A.L.A. (1) Ogawa,W.N. (1) Silva,M.P. da (1) de Deus,J.L. (1) Mais... Palavra-chave Nitric oxide (2) ATP (1) Calcium (1) Cell nucleus (1) Electrophysiology (1) Gene activity (1) Gene expression (1) Homeostasis (1) Ion channels (1) Leydig cells (1) Macromolecular transport (1) Magnocellular neurons (1) Measurement (1) NMDA receptors (1) NR2C subunits (1) Nuclear envelope (1) Nuclear pores (1) Nucleocytoplasmic transport (1) Nucleus tractus solitarius (1) Oxytocin (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Info:eu-repo/semantics/other (1) Ano 2018 (1) 2014 (1) 2005 (1) 1998 (1) País Brazil (4) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Effects of nitric oxide on magnocellular neurons of the supraoptic nucleus involve multiple mechanisms BJMBR Silva,M.P. da; Cedraz-Mercez,P.L.; Varanda,W.A.. Physiological evidence indicates that the supraoptic nucleus (SON) is an important region for integrating information related to homeostasis of body fluids. Located bilaterally to the optic chiasm, this nucleus is composed of magnocellular neurosecretory cells (MNCs) responsible for the synthesis and release of vasopressin and oxytocin to the neurohypophysis. At the cellular level, the control of vasopressin and oxytocin release is directly linked to the firing frequency of MNCs. In general, we can say that the excitability of these cells can be controlled via two distinct mechanisms: 1) the intrinsic membrane properties of the MNCs themselves and 2) synaptic input from circumventricular organs that contain osmosensitive neurons. It has also been... Tipo: Info:eu-repo/semantics/article Palavras-chave: Magnocellular neurons; Supraoptic nucleus; Vasopressin; Oxytocin; Nitric oxide; Homeostasis. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200090 Electrophysiological evidence for the presence of NR2C subunits of N-methyl-D-aspartate receptors in rat neurons of the nucleus tractus solitarius BJMBR Baptista,V.; Ogawa,W.N.; Aguiar,J.F.; Varanda,W.A.. The nucleus tractus solitarius (NTS) plays an important role in the control of autonomic reflex functions. Glutamate, acting on N-methyl-D-aspartate (NMDA) and non-NMDA ionotropic receptors, is the major neurotransmitter in this nucleus, and the relative contribution of each receptor to signal transmission is unclear. We have examined NMDA excitatory postsynaptic currents (NMDA-EPSCs) in the subpostremal NTS using the whole cell patch clamp technique on a transverse brainstem slice preparation. The NMDA-EPSCs were evoked by stimulation of the solitary tract over a range of membrane potentials. The NMDA-EPSCs, isolated pharmacologically, presented the characteristic outward rectification and were completely blocked by 50 µM DL-2-amino-5-phosphonopentanoic... Tipo: Info:eu-repo/semantics/other Palavras-chave: Nucleus tractus solitarius; Electrophysiology; Synapse; NMDA receptors; NR2C subunits. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100016 Nitric oxide modulates ATP-evoked currents in mouse Leydig cells BJMBR de Deus,J.L.; Dagostin,A.L.A.; Varanda,W.A.. Testosterone synthesis within Leydig cells is a calcium-dependent process. Intracellular calcium levels are regulated by different processes including ATP-activated P2X purinergic receptors, T-type Ca2+ channels modulated by the luteinizing hormone, and intracellular calcium storages recruited by a calcium-induced calcium release mechanism. On the other hand, nitric oxide (NO) is reported to have an inhibitory role in testosterone production. Based on these observations, we investigated the interaction between the purinergic and nitrergic systems in Leydig cells of adult mice. For this purpose, we recorded ATP-evoked currents in isolated Leydig cells using the whole cell patch clamp technique after treatment with L-NAME (300 μM and 1 mM), L-arginine (10,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Leydig cells; ATP; Purinergic receptor; Calcium; Nitric oxide; Whole cell patch clamp. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500603 Patch-clamp detection of macromolecular translocation along nuclear pores BJMBR Bustamante,J.O.; Varanda,W.A.. The present paper reviews the application of patch-clamp principles to the detection and measurement of macromolecular translocation along the nuclear pores. We demonstrate that the tight-seal 'gigaseal' between the pipette tip and the nuclear membrane is possible in the presence of fully operational nuclear pores. We show that the ability to form a gigaseal in nucleus-attached configurations does not mean that only the activity of channels from the outer membrane of the nuclear envelope can be detected. Instead, we show that, in the presence of fully operational nuclear pores, it is likely that the large-conductance ion channel activity recorded derives from the nuclear pores. We conclude the technical section with the suggestion that the best way to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Nuclear pores; Nuclear envelope; Cell nucleus; Nucleocytoplasmic transport; Macromolecular transport; Gene activity; Gene expression; Transcriptional control; Ion channels; Patch-clamp; Measurement. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300003 Registros recuperados: 4 Primeira ... 1 ... 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