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Anti-Leptospira spp and anti-Brucella spp antibodies in humans from rural area of Monte Negro municipality, state of Rondônia, Brazilian Western Amazon BJM
Aguiar,Daniel M.; Cavalcante,Guacyara T.; Camargo,Luis M. A.; Labruna,Marcelo B.; Vasconcellos,Silvio A.; Souza,Gisele O.; Gennari,Solange M..
Sera from 276 humans living in 71 farms located in Monte Negro Municipality, RO., Western Amazon, Brazil were examined for anti-Leptospira spp antibodies by Microscopic Agglutination Test and for anti-Brucella spp antibodies by Tube Agglutination Test. Leptospira spp antibodies were detected in 28 (10.2%) of them with at least one positive case in 23 farms (32.4%). The most frequent leptospira serovars were Patoc (46.7%), Autumnalis (10.0%) and Shermani (10.0%). The proportion of positive males (14.5%) were higher than females (5.0%; P <0.05) and the contact with river water presented association with Leptospira spp infection (OR: 27; P=0.01). A total of 04 (1.4%) humans reacted against Brucella antigens; three farms (4.2%) presented at least one...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human; Leptospirosis; Brucellosis; Amazon; Brazil.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100019
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First isolation of leptospires from dairy goats in Brazil BJM
Lilenbaum,Walter; Morais,Zenaide M.; Gonçales,Amane Paldês; Souza,Gisele O. de; Richtzenhain,Leonardo; Vasconcellos,Silvio A..
Leptospires have never been recovered from goats in Brazil. Serum samples were obtained from 248 goats from Rio de Janeiro and from the seroreactive animals, urine samples were collected and processed for Leptospira isolation. A total of 52 positive reactions were observed, corresponding to 20.9% of the samples. The most prevalent reactions were to serovars Hardjo (36.5%), Shermani (30.8%), Icterohaemorrhagiae (9.6%), Grippotyphosa (9.6%), Autumnalis (5.8%), Castellonis (3.8%) and Bratislava (3.8%). Two strains of Leptospira sp. were isolated, both in the same region, but from different flocks. Presumptive identification based on serologic methods suggests those strains to be from Grippotyphosa serogroup.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Goats; Leptospirosis; Leptospira sp.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300023
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Isolation of Leptospira santarosai, serovar guaricura from buffaloes (Bubalus bubalis) in Vale do Ribeira, São Paulo, Brazil BJM
Vasconcellos,Silvio A.; Oliveira,João C.F.; Morais,Zenáide M.; Baruselli,Pietro S.; Amaral,Renato; Pinheiro,Sônia R.; Ferreira,Fernando; Ferreira-Neto,José S.; Schönberg,Arno; Hartskeerl,Rudy A..
In April 1998 urine samples from adult female buffaloes were collected in a farm located in Registro, Vale do Ribeira, São Paulo State, Brazil. The urine samples obtained after furosemide injection were immediately transported to the laboratory in liquid modified EMJH medium and seeded, by the serial dilution technique, into Fletcher's or modified EMJH-0.2% agar, both of them with 5-fluorouracil 100mg/mL. The intraperitoneoum inoculation of 0.5 mL was also performed with each urine sample in young, adult hamsters (Mesocricetus auratus). All samples seeded directly in culture medium were contaminated. The hamsters did not show any sign of disease and were killed at the 21st post inoculation day. At this time kidney cultures of these animals were performed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospirosis; Serogroup Sejroe; Serovar guaricura; Buffalo.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400008
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