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Evaluation of bacterial diversity recovered from petroleum samples using different physical matrices BJM
Dellagnezze,Bruna Martins; Vasconcellos,Suzan Pantaroto de; Melo,Itamar Soares de; Santos Neto,Eugênio Vaz dos; Oliveira,Valéria Maia de.
ABSTRACT Unraveling the microbial diversity and its complexity in petroleum reservoir environments has been a challenge throughout the years. Despite the techniques developed in order to improve methodologies involving DNA extraction from crude oil, microbial enrichments using different culture conditions can be applied as a way to increase the recovery of DNA from environments with low cellular density for further microbiological analyses. This work aimed at the evaluation of different matrices (arenite, shale and polyurethane foam) as support materials for microbial growth and biofilm formation in enrichments using a biodegraded petroleum sample as inoculum in sulfate reducing condition. Subsequent microbial diversity characterization was carried out...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial diversity; Microbial enrichment; Petroleum reservoir; Physical support; 16S rRNA.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300712
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Halotolerant bacteria in the São Paulo Zoo composting process and their hydrolases and bioproducts BJM
Oliveira,Lilian C.G.; Ramos,Patricia Locosque; Marem,Alyne; Kondo,Marcia Y.; Rocha,Rafael C.S.; Bertolini,Thiago; Silveira,Marghuel A.V.; Cruz,João Batista da; Vasconcellos,Suzan Pantaroto de; Juliano,Luiz; Okamoto,Debora N..
Halophilic microorganisms are able to grow in the presence of salt and are also excellent source of enzymes and biotechnological products, such as exopolysaccharides (EPSs) and polyhydroxyalkanoates (PHAs). Salt-tolerant bacteria were screened in the Organic Composting Production Unit (OCPU) of São Paulo Zoological Park Foundation, which processes 4 ton/day of organic residues including plant matter from the Atlantic Rain Forest, animal manure and carcasses and mud from water treatment. Among the screened microorganisms, eight halotolerant bacteria grew at NaCl concentrations up to 4 M. These cultures were classified based on phylogenetic characteristics and comparative partial 16S rRNA gene sequence analysis as belonging to the genera Staphylococcus,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Halophilic; Protease; Lipase; Amylase; Cellulase.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200347
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