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Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil BJM
Vaucher,Rodrigo de Almeida; Dezen,Diogenes; Simonetti,Amauri Braga; Spilki,Fernando Rosado; Roehe,Paulo Michel.
Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bPI3V. The Brazilian isolates revealed a high degree of genomic when compared to SF4/32 prototype strain, within the recently proposed genotype A of bPIV-3.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine parainfluenza virus type 3; BPIV-3; Phylogenetic analysis.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400029
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Physiological responses of Rhamdia quelen (Siluriformes: Heptapteridae) to anesthesia with essential oils from two different chemotypes of Lippia alba Neotropical Ichthyology
Souza,Carine de Freitas; Baldissera,Matheus Dellaméa; Salbego,Joseânia; Lopes,Jane Mello; Vaucher,Rodrigo de Almeida; Mourão,Rosa Helena Veras; Caron,Braulio O.; Heinzmann,Berta Maria; Silva,Lenise Vargas Flôres da; Baldisserotto,Bernardo.
ABSTRACT The aim of this study was to evaluate if Lippia alba has different chemotypes according to the chemical composition of the essential oil (EO) considering collection site, and if the EO may have different effects on blood and plasma parameters in silver catfish, Rhamdia quelen, during and immediately after anesthesia. The citral (EO-C) and linalool (EO-L) chemotypes were identified, and both presented similar anesthetic effects for silver catfish. Fish were exposed to two concentrations of each EO, which induced slow and fast anesthesia (100 and 300 µL L-1, respectively). Blood ions did not change at any time of anesthesia induction and recovery and, therefore, the electrolyte balance was not altered. Blood gases oscillated through all exposure and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood gas; Cortisol; Glucose; Plasma ions; Silver Catfish.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252017000100203
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