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| Tokano,Dorismey Vieira; Kawaichi,Marisa Emiko; Venâncio,Emerson José; Vidotto,Marilda Carlos. |
| The aim of this work was to isolate, clone and characterize the iron uptake gene iutA from avian pathogenic E. coli (APEC). The iutA gene was isolated from the strain APEC 9, serotype O2:H9, which was cloned in the expression vector pET101/D-TOPO. The gene of 2.2 Kb was sequenced (AY602767, which showed high similarity to the iutA gene from three plasmids, two from APEC, pAPEC-02-ColV (AY545598.4) and pTJ100 (AY553855.1), and one from a human invasive E. coli strain, the pColV K30. The recombinant protein IutA was over expressed in E. coli BL21(DE-3) and was solubilized with urea and purified by Ni-NTA column. This method produced a relatively high yield of r-IutA of approximately 74kDa, which was used to produce the antibody anti-IutA. This anti-IutA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Avian Escherichia coli; Iron uptake; Aerobactin receptor; Virulence factor; IutA gene. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300006 |
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| Watanabe,Maria Angelica Ehara; Miquelão,Ana Karina Melim Benthien; Oliveira,Carlos Eduardo Coral de; Oliveira,Karen Brajão de; Nasser,Thiago Franco; Aoki,Mateus Nóbrega; Suzuki,Patrícia Sayuri; Amarante,Marla Karine; Venâncio,Emerson José; Azevedo,Elbens Marcos Minoreli de. |
| The purpose of the present study was to evaluate the sexual transmission of GBV-C/HGV, through RNA detection in cervicovaginal smears. Therefore the GBV-C/HGV RNA in cervicovaginal smears from apparently healthy women was investigated using routine proceedings for prophylactic screening to cervical cancer. GBV-C/HGV RNA was detected by reverse transcriptase and polymerase chain reaction (RT-PCR). Only one woman presented co-infection with human papilloma virus (HPV). The GBV-C/HGV RNA was detected in 13/73 (17.57%) healthy women and it's prevalence in participating women between 28-43 years old was 53.85%. No association was found with GBV-C/HGV for the age of first sexual intercourse and number of pregnancies. In GBV-C/HGV RNA positive women, 69.23% were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: GBV-C/HGV RNA; Healthy women; Cervicovaginal smears. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500007 |
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