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Registros recuperados: 32
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Current Methods for Seafood Authenticity Testing in Europe: is there a Need for Harmonisation? ArchiMer
Griffiths, Andrew M.; Sotelo, Carmen G.; Mendes, Rogerio; Perez-martin, Ricardo I.; Schroeder, Ute; Shorten, Marc; Silva, Helena A.; Verrez-bagnis, Veronique; Mariani, Stefano.
Mislabelling of food products has recently received a great deal of public scrutiny, but it remains unclear exactly what methods are being utilised in laboratories testing the authenticity of foods. In order to gain insight into the specific area of the analysis of seafood, a questionnaire focusing on the taxonomic groups typically analysed and the techniques utilised was sent to over one hundred accredited laboratories across the UK, Ireland, Spain, Portugal, France and Germany. Forty-five responded positively, demonstrating significant differences in both the species analysed and methods utilised among the countries included in the survey. Indeed, a diversity of methods was employed across laboratories and efforts to harmonise and/or standardise testing...
Tipo: Text Palavras-chave: Food testing; Forensically informative nucleotide sequencing; Species identification; DNA barcoding; Fisheries.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00188/29918/28383.pdf
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Prevalence, Molecular Identification, and Risk Factors for Cryptosporidium Infection in Edible Marine Fish: A Survey Across Sea Areas Surrounding France ArchiMer
Certad, Gabriela; Follet, Jérôme; Gantois, Nausicaa; Hammouma-ghelboun, Ourida; Guyot, Karine; Benamrouz-vanneste, Sadia; Fréalle, Emilie; Seesao, Yuwalee; Delaire, Baptiste; Creusy, Colette; Even, Gaël; Verrez-bagnis, Veronique; Ryan, Una; Gay, Mélanie; Aliouat-denis, Cécile; Viscogliosi, Eric.
Cryptosporidium, a zoonotic pathogen, is able to infect a wide range of hosts including wild and domestic animals, and humans. Although it is well known that some parasites are both fish pathogens and recognized agents of zoonosis with a public health impact, little information is available concerning the prevalence of Cryptosporidium in wild aquatic environments. To evaluate the prevalence of Cryptosporidium spp. in commercially important edible marine fish in different European seas (English channel, North sea, Bay of Biscay, Celtic sea and Mediterranean sea), 1,853 specimens were collected as part of two surveys. Nested PCR followed by sequence analysis at the 18S rRNA gene locus was used to identify Cryptosporidium spp. The overall prevalence of...
Tipo: Text Palavras-chave: Cryptosporidium; Edible marine fish; 18S rRNA gene; Gp60; Molecular epidemiology; Phylogeny; Novel genotypes; European seas.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00497/60864/64198.pdf
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Development of a qPCR Method for the Identification and Quantification of Two Closely Related Tuna Species, Bigeye Tuna ( Thunnus obesus ) and Yellowfin Tuna ( Thunnus albacares ), in Canned Tuna ArchiMer
Bojolly, Daline; Doyen, Perine; Le Fur, Bruno; Christaki, Urania; Verrez-bagnis, Veronique; Grard, Thierry.
Bigeye tuna (Thunnus obesus) and yellowfin tuna (Thunnus albacares) are among the most widely used tuna species for canning purposes. Not only substitution but also mixing of tuna species is prohibited by the European regulation for canned tuna products. However, as juveniles of bigeye and yellowfin tunas are very difficult to distinguish, unintentional substitutions may occur during the canning process. In this study, two mitochondrial markers from NADH dehydrogenase subunit 2 and cytochrome c oxidase subunit II genes were used to identify bigeye tuna and yellowfin tuna, respectively, utilizing TaqMan qPCR methodology. Two different qPCR-based methods were developed to quantify the percentage of flesh of each species used for can processing. The first one...
Tipo: Text Palavras-chave: Tuna; Authentication; Quantification; Canned products; TaqMan; QPCR; Species identification; Bigeye tuna (Thunnus obesus); Yellowfin tuna (Thunnus albacares).
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00371/48217/48403.pdf
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Identification of skipjack tuna juveniles based on DNA control region sequences and potential spawning area around reunion island ArchiMer
Nikolic, Natacha; Jerome, Marc; Fonteneau, Alain; Evano, Hugues; Verrez-bagnis, Veronique.
In this study, we confirmed that DNA control region can be used to identify potential spawning area from pelagic juveniles species. We used the mitochondrial DNA control region to identify tuna juveniles predated by common dolphinfish and sampled in surface waters around Reunion Island. These were skipjack juveniles at an early stage (small length and young age). The biological results confirmed the species identification based on DNA analysis. Catch data by longline reveal a higher number of skipjack from October to December around Reunion Island corresponding to the period of our skipjack juvenile sampling
Tipo: Text Palavras-chave: Skipjack tuna; Reunion island; DNA; Age; Juvenile.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00299/40993/42657.pdf
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Tuna labels matter in Europe: Mislabelling rates in different tuna products ArchiMer
Sotelo, Carmen G.; Velasco, Amaya; Perez-martin, Ricardo, I; Kappel, Kristina; Schroeder, Ute; Verrez-bagnis, Veronique; Jerome, Marc; Mendes, Rogerio; Silva, Helena; Mariani, Stefano; Griffiths, Andrew.
Tuna fisheries and processing represent economic activities of paramount importance around the world. Most of these products are traded for human consumption and in general are highly demanded commodities. However, not all tuna products achieve the same market price, some consumers are willing to pay a huge amount of money for certain species (i.e. Japanese market for Bluefin tuna) while other species are rather affordable (i.e. Skipjack tuna), therefore mislabelling has been observed frequently. We collected and analysed 545 tuna samples in six European countries, including fresh, frozen and canned products, and we have investigated whether or not these products were correctly labelled under European and national legislations. We found an overall...
Tipo: Text
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00440/55137/56604.pdf
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Effects of high pressure on texture and microstructure of sea bass (Dicentrarchus labrax L.) fillets ArchiMer
Cheret, Romuald; Chapleau, Nicolas; Delbarre Ladrat, Christine; Verrez-bagnis, Veronique; De Lamballerie, Marie.
High pressure is an innovative non-thermal food-preservation technology. We studied the effect of high-pressure treatment up-to 500 MPa for 5 min on physical characteristics of sea bass fillets after 0, 7, and 14 d of refrigerated storage.. Color results exhibited an increase of lightness and a slight change of hue, which might be imperceptible in cooked fish. High-pressure treatment induced a decrease of exudation and water-holding capacity Pressure treatment above 300 MPa provoked higher fish hardness after storage than in untreated sample, proving the ability of high pressure to improve textural, quality of chilled, stored fish fillet. These assessments were corroborated with microstructure observations. We showed that high-pressure treatment at 500 MPa...
Tipo: Text Palavras-chave: Preservation; Microstructure; Texture profile analysis; High pressure; Fish.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-734.pdf
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Monitoring of four DNA extraction methods upstream of high-throughput sequencing of Anisakidae nematodes ArchiMer
Seesao, Y.; Audebert, C.; Verrez-bagnis, Veronique; Merlin, S.; Jerome, Marc; Viscogliosi, E.; Dei-cas, E.; Aliouat-denis, C. M.; Gay, Melanie.
Different methods were evaluated to extract DNA from pooled nematodes belonging to Anisakis, Contracaecum, Pseudoterranova and Hysterothylacium genera isolated from edible fish. Pooled DNA extraction is the first and compulsory step to allow the identification of a large number of samples through high-throughput DNA sequencing with drastic time and cost reductions.
Tipo: Text Palavras-chave: Anisakidae; High-throughput sequencing; Nematode; Pooled DNA extraction; Marine fish; COX2.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00189/30020/28607.pdf
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In vitro proteolysis of myofibrillar and sarcoplasmic proteins of European sea bass (Dicentrarchus Labrax L) by an endogenous m-calpain ArchiMer
Verrez-bagnis, Veronique; Delbarre Ladrat, Christine; Noel, Joelle; Fleurence, Joel.
The effects of m-calpain isolated from the skeletal muscle of sea bass on sarcoplasmic and myofibrillar proteins isolated from the same tissue were examined in vitro. Incubation of sarcoplasmic proteins with m-calpain resulted in only a slight decrease (0.7 kDa) in the molecular weight (MW) of a 26.5 kDa protein. Degradation of myofibrils, monitored by quantification of TCA-soluble peptides generated, resulted in the maximum amount of peptides being generated after 1 h of incubation at 25degreesC. Noticeable modifications in the SDS-PAGE profile of digested myofibrils were observed, including partial denaturation of myosin heavy chain and the release of tropomyosin, similar to69 and similar to27 kDa doublet bands and a few polypeptides of MW lower than 20...
Tipo: Text Palavras-chave: Dicentrarchus labrax L; Proteolysis; Sarcoplasmic protein; Calpain; Myofibrillar protein.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-1108.pdf
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Evaluation, signalling and willingness to pay for traceability. A cross-national comparison ArchiMer
Calvo Dopico, D.; Mendes, R.; Silva, H.a.; Verrez-bagnis, Veronique; Pérez-martín, R.; Sotelo, C.g..
This study aims to investigate the benefits associated with traceability and its relationship to quality. Market research has been conducted in Spain, Portugal, France, UK and Germany. The results show that the expected benefits for consumers related to traceability focus on food safety and quality. Relevant information about traceability should focus on the intrinsic quality attributes highly valued by consumers and food safety. Among them, it highlights the origin, the value of which has been highly appreciated by most consumers in different countries. As regards signalling, consumers rate labelling very favourably, which would have to offer all relevant information on the intrinsic quality attributes. The implementation of technology (e.g. QR) could...
Tipo: Text Palavras-chave: Labelling; Signal; Consumer behaviour; Traceability; Quality.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00349/46017/46901.pdf
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Low mislabeling rates indicate marked improvements in European seafood market operations ArchiMer
Mariani, Stefano; Griffiths, Andrew M.; Velasco, Amaya; Kappel, Kristina; Jerome, Marc; Perez-martin, Ricardo I.; Schroeder, Ute; Verrez-bagnis, Veronique; Silva, Helena; Vandamme, Sara G.; Boufana, Belgees; Mendes, Rogerio; Shorten, Marc; Smith, Cat; Hankard, Elizabeth; Hook, Samantha A.; Weymer, Alice S.; Gunning, Daryl; Sotelo, Carmen G..
Over the span of a decade, genetic identification methods have progressively exposed the inadequacies of the seafood supply chain, revealing previously unrecognized levels of seafood fraud, raising awareness among the public, and serving as a warning to industry that malpractice will be detected. Here we present the outcome of the latest and largest multi-species, transnational survey of fish labeling accuracy to date, which demonstrates an apparent sudden reduction of seafood mislabeling in Europe. We argue that recent efforts in legislation, governance, and outreach have had a positive impact on industry regulation. Coordinated, technology-based, policy-oriented actions can play a pivotal role in shaping a transparent, sustainable global seafood market...
Tipo: Text
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00302/41323/40517.pdf
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Proteolytic potential in white muscle of sea bass (Dicentrarchus labrax L.) during post mortem storage on ice: time-dependent changes in the activity of the components of the calpain system ArchiMer
Delbarre Ladrat, Christine; Verrez-bagnis, Veronique; Noel, Joelle; Fleurence, Joel.
The variations in the amounts of milli-calpain and its specific inhibitor in the white muscle of sea bass (Dicentrarchus labrax) during storage at 4 degreesC for up to 7 days were determined after separation by hydrophobic chromatography on a Phenyl Sepharose vel. There was a significant decline in post-slaughter m-calpain activity with an important inter-individual variability in the rate of decrease of the total activity. In contrast with the calpastatin of mammalian post mortem muscles, calpastatin remained constant within fish muscles after death. The initial levels of protease and inhibitor activities, and their behaviour through post mortem storage. are discussed and implications for the mechanism of tenderisation of fish muscle are suggested. (C)...
Tipo: Text Palavras-chave: Proteolysis; Post mortem; Fish muscle; Calpain; Calpastatin; Neutral calcium dependent protease.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-1683.pdf
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Suite de l'étude entreprise sur la texture du surimi et gel de surimi (Kamaboko) de chinchard (Trachurus trachurus) ArchiMer
Verrez-bagnis, Veronique.
A la question : Qu'est-ce que le surimi ?, l'introduction du rapport annuel d'activité de juin 1985 "Essais préliminaires et premleres études de l'influence des paramètres technologiques sur la texture du surimi et gel de surimi de chinchard (Trachurus trachurus)" a tenté d'apporter une réponse. Après le détail de la fabrication du surimi employée ainsi que les résultats de quelques essais préliminaires permettant de choisir les méthodes de mesures adéquates, nous avions abordé la premiere grande partie du travail - l'optimisation des conditions technologiques - et indiqué les prémices d'une étude sur la microstructure du surimi et du kamaboko. Actuellement la première partie de l'étude est terminée et deux articles "Texture du kamaboko de chinchard...
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Ano: 1986 URL: http://archimer.ifremer.fr/doc/00125/23671/21520.pdf
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Maîtrise de la texture des produits à base de surimi ArchiMer
Han Ching, Lucay; Verrez-bagnis, Veronique.
Le surimi est une fraction protéique stabilisée, obtenue a partir de muscles de poissons. II peut être fabriqué dans des usines à bord des bateaux ou à terre.
Tipo: Text
Ano: 1990 URL: http://archimer.ifremer.fr/doc/00057/16809/14272.pdf
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Calpain and cathepsin activities in post mortem fish and meat muscles ArchiMer
Cheret, Romuald; Delbarre Ladrat, Christine; De Lamballerie Anton, Marie; Verrez-bagnis, Veronique.
Post mortem tenderization is one of the most unfavourable quality changes in fish muscle and this contrasts with muscle of mammalian meats. The tenderization can be partly attributed to the acid lysosomal cathepsins and cytosolic neutral calcium-activated calpains. In this study, these proteases from fish and bovine muscles were quantified and compared. The cathepsin B and L activities were in more important amounts in sea bass white muscle than in bovine muscle. On the other hand, cathepsin D activity was 1.4 times higher in meat that in fish muscle, while cathepsin H was negligible in both muscles. Calpain activities were similar in both types of muscle. Moreover, calpastatin (calpain endogenous inhibitor) level is 3.9 times higher in sea bass white...
Tipo: Text Palavras-chave: Meat; Fish; Protease; Cathepsin; Calpain.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3646.pdf
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Petromyzon marinus (Petromyzontidae), an unusual host for helminth parasites in western Europe ArchiMer
Gerard, Claudia; Verrez-bagnis, Veronique; Jerome, Marc; Lasne, Emilien.
The sea lamprey Petromyzon marinus, which is among the most phylogenetically ancient vertebrates, is a hematophagous ectoparasite that feeds on vertebrates and is considered vulnerable in Europe but is a pest in the North American Great Lakes. We conducted a literature review of helminth parasites of Petromyzon marinus and investigated postmetamorphic lampreys sampled in rivers and northeast Atlantic coastal waters (western France) during spawning migration. Based on the literature review, 16 helminth taxa have been recorded in Petromyzon marinus, among them 14 in North America but only 2 in Europe, with no species in common between these areas. Specific parasites are lacking, and helminth parasites recorded in Petromyzon marinus are mostly opportunistic...
Tipo: Text Palavras-chave: Sea lamprey; Helminth parasites; Unusual host; Accidental ingestion; Anisakis simplex sensu strict; Nematode.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00268/37964/36040.pdf
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Genetic catalogue, biological reference collections and online database of European marine fishes ArchiMer
Bautista, José M.; Verrez-bagnis, Veronique; Kourti, Naouma.
Taxonomy and systematics of most animal species have been described in sufficient detail to permit the classification of practically any organism and, in particular, may be of use in the identification of the species and subspecies for the fish trade and ecological concerns regarding fisheries. However, before FishTrace started there was a lack of fast reference tools that hindered the efficient identification and differentiation of teleosts required in fisheries management, biological and ecological research, and human consumption. This problem could affect several of the socio-economic activities related to Fisheries Policies and therefore, the constitution of a database for identification of fish species of commercial, ecological and zoological interest...
Tipo: Text Palavras-chave: Database; Fish; Genetics.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/rapport-6595.pdf
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Surimi de chinchard ( Trachurus trachurus L. ) - Influence de conditions technologiques sur des caractéristiques de texture du produit après traitement thermique ArchiMer
Verrez-bagnis, Veronique.
Surimi, washed fish mince after grinding with salt, has a firm and elastic gel texture or “ashi” after thermal processing. The major objectives of this study on horse mackerel (Trachurus trachurus) surimi are to optimize the technological conditions in the aim to obtain the best ashi, to determine the influence of frozen storage and of egg white and potata starch addition, and to establish the preventive effect of incorporation into surimi of different combinations of cryoprotectants and NaC1. Gel texture is, by order importance, dependent on cooking temperature, length of setting and cooking and length of grinding of surimi with texture additives after thawing. Changes of gelling properties after 6 months at - 30°C of frozen st orage are limited. The...
Tipo: Text Palavras-chave: Fish; Horse mackerel (Trachurus trachurus L.); Surimi; Kamaboko; Myofibrillar proteins; Gelation; Thermal coagulation; Texture; Optimisation method; Technological treatments; Cryoprotectors; Texture ingredients; Microscopy; Water retention capacity.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/00134/24507/22535.pdf
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Primers and polymerase chain reaction conditions for DNA barcoding teleost fish based on the mitochondrial cytochrome b and nuclear rhodopsin genes ArchiMer
Sevilla, Rafael G.; Diez, Amalia; Noren, Michael; Mouchel, Olivier; Jerome, Marc; Verrez-bagnis, Veronique; Van Pelt, Hilde; Favre Krey, Laurence; Krey, Grigorios; Bautista, José M..
This report describes a set of 21 polymerase chain reaction primers and amplification conditions developed to barcode practically any teleost fish species according to their mitochondrial cytochrome b and nuclear rhodopsin gene sequences. The method was successfully tested in more than 200 marine fish species comprising the main Actinopterygii family groups. When used in phylogenetic analyses, its combination of two genes with different evolutionary rates serves to identify fish at the species level. We provide a flow diagram indicating our validated polymerase chain reaction amplification conditions for barcoding and species identification applications as well as population structure or haplotyping analyses, adaptable to high-throughput analyses.
Tipo: Text Palavras-chave: Teleost; Rhodopsin; PCR primers; Mitochondrial cytochrome b; Fish; DNA barcoding.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3034.pdf
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Comparative genomics reveals a widespread distribution of an exopolysaccharide biosynthesis gene cluster among Vibrionaceae ArchiMer
Lebellenger, Lou; Verrez-bagnis, Veronique; Passerini, Delphine; Delbarre Ladrat, Christine.
Objectives The eps locus in Vibrio diabolicus is involved in the production of the biotechnologically valuable HE800 EPS. In this study, the distribution and diversity of similar eps gene clusters across Vibrionaceae and its variability in relation to phylogenetic relationship were investigated. The aim was to provide a better knowledge of the eps gene cluster importance and to facilitate discovery of new EPS with potent interesting bioactivities. Results Seventy percent of the 103 genome sequences examined display such an eps locus with a high level of synteny. However, genetic divergence was found inside some monophyletic clades or even between some strains of the same species. It includes gene insertions, truncations, and deletions. Comparative analysis...
Tipo: Text Palavras-chave: Vibrionaceae; Exopolysaccharide; Genetic biodiversity; Biosynthesis; Vibrio diabolicus; HE800 EPS.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00424/53536/54392.pdf
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In vitro proteolysis of myofibrillar and sarcoplasmic proteins of white muscle of sea bass (Dicentrarchus labrax L.): effects of cathepsins B, D and L ArchiMer
Delbarre Ladrat, Christine; Verrez-bagnis, Veronique; Noel, Joelle; Fleurence, Joel.
The purpose of this study was to obtain additional information regarding proteolysis mechanisms and disorganization of fish myofibrils resulting in a loss of flesh quality. The ability of cathepsins to degrade in vitro myofibrillar and sarcoplasmic proteins from fish muscle was investigated in order to explain their role in post mortem softening. This led to the identification of substrates of the enzymes. Cathepsins degraded myosin heavy chain and a-actinin. Tropomyosin and actin were only susceptible to the action of cathepsin L. Troponin T (assumed 32 kDa component) was resistant only to the action of cathepsin D. Desmin was degraded by cathepsins B and L. Slight changes of some other myofibrillar or cytosolic proteins were also observed (creatine...
Tipo: Text Palavras-chave: Post mortem degradation; Fish muscle proteins; Cathepsins; In vitro proteolysis.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-1684.pdf
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