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| Pereira,M.A.A.; Nowosh,V.; Suffys,P.N.; Queiroz,G.B.; Silva,K.M.O.; Lourenço,M.C.S.; Vicente,A.C.P.; Fontes,A.N.B.; Morgado,S.; Neves,R.C.S.M.. |
| ABSTRACT Canine Leproid Granuloma Syndrome (CLGS), also known as canine leprosy, is a cutaneous nodular infectious disease caused by Mycobacterium sp.. Despite being reported worldwide, it is still quite unknown and underdiagnosed. Diagnosis may be achieved by cytopathology or histopathology of skin lesions, but identification of the infectious agent is complex, since bacterial in vitro growth is not possible, relying upon molecular techniques such as PCR to confirm Mycobacterium DNA in the sample. We report a CLGS case in Niteroi, Rio de Janeiro state, Brazil, diagnosed by cytopathology and submitted to molecular identification of the agent. PCR amplification of hsp65 gene was performed and revealed 100% genetic homology to M. murphy strain. This is the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CLGS; Hsp65 sequence; Isolate Metcalfe; Molecular methods. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000601699 |
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| Câmara,C.C.; Oriá,R.B.; Felismino,T.C.; Silva,A.P. da; Silva,M.A.M. da; Alcântara,J.V.A.; Costa,S.B.C.; Vicente,A.C.P.; Teixeira-Santos,T.J.; Castro-Costa,C.M. de. |
| The objective of the present study was to describe motor behavioral changes in association with histopathological and hematological findings in Wistar rats inoculated intravenously with human T-cell lymphotropic virus type 1 (HTLV-1)-infected MT2 cells. Twenty-five 4-month-old male rats were inoculated with HTLV-1-infected MT2 cells and 13 control rats were inoculated with normal human lymphocytes. The behavior of the rats was observed before and 5, 10, 15, and 20 months after inoculation during a 30-min/rat testing time for 5 consecutive days. During each of 4 periods, a subset of rats was randomly chosen to be sacrificed in order to harvest the spinal cord for histopathological analysis and to obtain blood for serological and molecular studies.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Motor behaviors; HTLV-1-infected MT2 cells; Wistar rats; Experimental myelopathy. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000700008 |
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