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Supraorganized collagen enhances Schwann cell reactivity and organization in vitro BJMBR
Maturana,L.G.; Zanon,R.G.; Pierucci,A.; Vidal,B.C.; Oliveira,A.L.R..
We investigated the reactivity and expression of basal lamina collagen by Schwann cells (SCs) cultivated on a supraorganized bovine-derived collagen substrate. SC cultures were obtained from sciatic nerves of neonatal Sprague-Dawley rats and seeded on 24-well culture plates containing collagen substrate. The homogeneity of the cultures was evaluated with an SC marker antibody (anti-S-100). After 1 week, the cultures were fixed and processed for immunocytochemistry by using antibodies against type IV collagen, S-100 and p75NTR (pan neurotrophin receptor) and for scanning electron microscopy (SEM). Positive labeling with antibodies to the cited molecules was observed, indicating that the collagen substrate stimulates SC alignment and adhesion (collagen IV...
Tipo: Info:eu-repo/semantics/article Palavras-chave: S-100; P75NTR; Type IV collagen; Collagen; Cell culture; Supraorganized collagen.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700011
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