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Isolation and Identification of a Potential Amylolytic Probiotic Bacterium from the Gut of Jundiá Catfish, Rhamdia quelen. BABT
Poletto,Tatiana Vieira; Vieira,Cleide Rosana Werneck; Silva,Carlos Peres; Fracalossi,Debora Machado.
ABSTRACT This study aimed to isolate a potential probiotic amylolytic strain from the gut of jundiá catfish to improve carbohydrate digestibility in fish. Two of 31 strains isolated from the foregut of Rhamdia quelen were able to grow on starch-agar medium and were considered amylolytic. The strain that presented higher amylolytic potential, based on a qualitative amylase assay, was chosen. The strain was phenotypically characterized and analysed to determine bile and pH tolerance and extracellular quantitative amylase activity. The probiotic candidate, identified as Aeromonas veronii, showed the ability to survive stresses from a range of pH and bile salt conditions and secreted an interesting enzymatic profile, which may exhibit a synergistic effect when...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzymes; Probiotics; Aquaculture; Intestinal microbiology; Aeromonas veronii.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100323
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Evaluation of the PetrifilmTM EB and TEMPO® EB systems with ISO 21528-2:2004 method for the count of Enterobacteriaceae in milk BJM
Cirolini,Andréia; Miotto,Marília; Machado,Fernanda Morgana; Silva,Helen Silvestre da; Ogliari,Paulo José; Vieira,Cleide Rosana Werneck.
The development of alternative microbiological techniques is driven by the necessity to meet the current needs to deliver rapid results in the manufacturing process of foods, but it is important that these methods be evaluated for each application. The objective of the present study was to assess the PetrifilmTM EB and the TEMPO® EB systems with ISO 21528-2:2004 for the count of Enterobacteriaceae in pasteurized and UHT milk samples. We analyzed the microflora of 141 pasteurized milk samples, 15 samples of artificially contaminated pasteurized milk and 15 samples of artificially contaminated UHT milk. Investigation of the method PetrifilmTM EB and ISO 21528:2 regression analysis showed a high correlation in the samples, r = 0.90 for the microflora of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterobacteriaceae; Milk; Alternative methods; ISO 21528-2:2004; Count.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300016
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Métodos alternativos para contagem de micro-organismos em carcaças suínas Ciência Rural
Cattani,Cristhiane Stecanella de Oliveira; Fuchs,Pedro Ivo Pinheiro; Cirolini,Andréia; Vieira,Cleide Rosana Werneck.
Micro-organismos estão presentes em toda a cadeia de processamento da carne, desde a matéria-prima até o produto final e compete aos programas de qualidade industrial verificar se existe contaminação na matéria-prima e nos produtos finais para, dessa forma, garantir um produto seguro ao consumidor. Para tanto, faz-se necessário utilizar metodologias alternativas à convencional na rotina diagnóstica dos laboratórios para obtenção de resultados confiáveis e em menor tempo possível. Com objetivo de verificar e comparar a correlação e o tempo de processamento, da metodologia convencional com as metodologias alternativas do sistema PetrifilmTM (3M) e sistema TEMPO® (bioMeriéux), foi realizada contagem de micro-organismos mesófilos, de enterobactérias e de...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Métodos; Carcaças; Suínos; Micro-organismos.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000600014
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Chemical composition and antimicrobial activity of essential oils from selected herbs cultivated in the South of Brazil against food spoilage and foodborne pathogens Ciência Rural
Silveira,Sheila Mello da; Cunha Júnior,Anildo; Scheuermann,Gerson Neudí; Secchi,Fábio Luiz; Vieira,Cleide Rosana Werneck.
The chemical composition of 10 selected plant essential oils obtained by steam distillation was determined by GC and GC/MS. The antimicrobial activity of the essential oils was screened against 12 important food-related bacterial strains by agar disc-diffusion assay. MIC and MBC were determined for the essential oils that presented the highest activity in the agar disc-diffusion test. The most active essential oils against the tested bacteria were, in descending order, lemongrass (Cymbopogon flexuosus), basil (Ocimum basilicum), oregano (Origanum vulgare), cinnamon leaf (Cinnamomum zeylanicum), and laurel (Laurus nobilis). Except for S. Typhimurium, the tested bateria were inhibited at MIC values lower or equal to 0.62mg mL-1 by lemongrass (Cymbopogon...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Essential oils; Chemical composition; Phytotherapic agents; Antibacterial activity; Food pathogens.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000700026
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