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| Regua-Mangia,Adriana Hamond; Gomes,Tânia Aparecida Tardelli; Andrade,João Ramos Costa; Vieira,Mônica Aparecida Midolli; Gonzalez,Alice Gonçalves Martins; Zahner,Viviane; Irino,Kinue; Teixeira,Lúcia Martins. |
| In the present study, 47 enteropathogenic Escherichia coli strains identified according to serotyping, presence of eae, bfp and EAF sequences, adherence phenotype and ability to induce attaching-effacing lesions were analyzed by pulsed-field gel electrophoresis (PFGE), multilocus enzyme electrophoresis (MLEE), and the presence of LEE genes (eae, espA, espB, tir) as well as the respective alleles. Amplification of LEE genes subtypes revealed 18 different pathotypes. Typing of the eae gene showed that most strains contained nontypable intimin (42%) followed by beta (35%), gamma and alpha genes (12% each). PFGE analysis revealed a variable degree of polymorphism among isolates and, in general, no clear correlation was observed among PFGE profiles and the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enteropathogenic Escherichia coli; Genetic diversity; Virulence markers. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500013 |
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| Liberatore,Ana Maria Alvim; Tomita,Sandra Kimie; Vieira,Mônica Aparecida Midolli; Toti Jr.,Cyro; Heckmaier,João; Gomes,Tânia Aparecida Tardelli. |
| The virulence attributes of 56 Escherichia coli strains isolated from sick horses (secretions of uterine cervices; gastrointestinal and lung fragments of necropsy; diarrheic feces, and tracheal washings) was examined by determining their adherence pattern to HeLa cells and searching for the presence of virulence genes of the various E. coli pathotypes. Two non-adherent strains presented astA, which encodes the enteroaggregative E. coli heat-stable toxin. Twenty-seven strains (48.2%) adhered to HeLa cells, 21 (77.8%) of which presented the aggregative adherence pattern (AA) that characterize the Enteroaggregative E. coli pathotype (EAEC). Nine of the strains presenting AA were isolated from secretions of uterine cervix, including one carrying virulence... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Horse; Escherichia coli; Aggregative adherence; Virulence. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100003 |
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| Sanches,Lilian Aparecida; Gomes,Marcelo da Silva; Teixeira,Rodrigo Hidalgo Friciello; Cunha,Marcos Paulo Vieira; Oliveira,Maria Gabriela Xavier de; Vieira,Mônica Aparecida Midolli; Gomes,Tânia Aparecida Tardelli; Knobl,Terezinha. |
| ABSTRACT Psittacine birds have been identified as reservoirs of diarrheagenic Escherichia coli, a subset of pathogens associated with mortality of children in tropical countries. The role of other orders of birds as source of infection is unclear. The aim of this study was to perform the molecular diagnosis of infection with diarrheagenic E. coli in 10 different orders of captive wild birds in the state of São Paulo, Brazil. Fecal samples were analyzed from 516 birds belonging to 10 orders: Accipitriformes, Anseriformes, Columbiformes, Falconiformes, Galliformes, Passeriformes, Pelecaniformes, Piciformes, Psittaciformes and Strigiformes. After isolation, 401 E. coli strains were subjected to multiplex PCR system with amplification of genes eae and bfp... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diarrheagenic E. coli; Atypical EPEC; STEC; Avian diseases. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400760 |
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| Knöbl,Terezinha; Gomes,Tânia Aparecida Tardelli; Vieira,Mônica Aparecida Midolli; Ferreira,Fernando; Bottino,José Américo; Ferreira,Antônio José Piantino. |
| Three hundred and fifty strains of E. coli isolated from septicemic poultry from seven states of Brazil were examined for presence of nine adhesion-encoding genes, hemagglutination and adherence to chicken tracheal cells (in vitro). Analysis of the strains by colony hybridization tests demonstrated that 93.7% of the isolates were fim +, 17% pap+ and 5.7% were sfa+. The mannose sensitive fimbriae occur with similar frequency in APEC isolated from all Brazilians states, while significant differences among pap and sfa genes distributions were observed. The results showed that 0.85% and 0.28% of APEC were positive for genes that encoded enteroaggregative adhesins and EPEC adherence factor, respectively. None of APEC was positive for DA, afa, Bfp and Eae... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: APEC; Escherichia coli; Adhesins; Colibacillosis; Poultry. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300033 |
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