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Single-channel response of hamster oocytes to ertilization with homologous spermatozoa Biocell
Ituarte,Leonor M.E.; Viera,Teresa B.; Saldeña,Teobaldo A.; De Rosas,Juan C.; Fóscolo,Mabel; Ibáñez,Jorge E.; Saraví,Fernando D..
Electrophysiological events occur early after fertilization, along with changes in intracellular Ca2+ concentration . Passive electrical parameters were determined in golden hamster oocytes by whole cell patch-clamp method. In separate experiments the effect of 4-aminopyridine on resting oocytes was tested. The single-channel patch clamp configuration was employed to assess the electrical response to fertilization with homologous sperm. Structure of oocytes submitted to patch clamp was evaluated with scanning electron microscopy and found to be preserved. Oocyte diameter was 70.2 ±  2.2 µm; their resting parameters were: membrane potential 23.8 ±  0.8 mV; total membrane specific resistance 519.1 ±  94.6 Ω.cm², and specific capacity 0.99 ±  0.03...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 4-Aminopyridine; Fertilization; Hamster oocyte; Patch-clamp; Membrane current.
Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100007
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