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Universal protocol for generating 100bp size standard for endless usage Electron. J. Biotechnol.
Natarajan,Chandrasekhar; Vijayan,Neetha N; Vaidyan,Linda Koshy; Mathew,Anila; Srinivas,Lekshmy; Banerjee,Moinak.
Developing countries are facing severe bottlenecks in the technological advancement in biotechnology, due to restrictions imposed by patent protected products and protocols. This calls for designing of simple and cost-effective alternatives for the indispensable products like DNA molecular weight markers. We demonstrate a novel, rapid and cost-effective method of making in-house 100bp ladder for routine use. In our method we use a single forward primer and five reverse primers designed on the backbone sequence of a commonly used vector template. These primers are used at a universal annealing temperature to amplify ten DNA fragments of accurate size ranging from 100bp to 1000bp. Our PCR-based method can provide size standards for an endless usage.
Tipo: Journal article Palavras-chave: 100bp ladder; Cost effective; DNA size standards; Endless usage.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200015
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